Figure 3

Linear correlation of UV _{488 nm} versus EGFP concentration. EGFP was diluted from 0.1 mg/ml to 0.6 mg/ml in MARS buffer A in 0.1-mg/ml increments, generating 7 concentration points. The UV absorbance of 200 μl of diluted sample was measured, ranging from 400 nm to 600 nm, on an ELISA reader. (A) Blank (MARS buffer A), (B) 0.1 mg/ml (20 μg), (C) 0.2 mg/ml (40 μg), (D) 0.3 mg/ml (60 μg), (E) 0.4 mg/ml (80 μg), (F) 0.5 mg/ml (100 μg), and (G) 0.6 mg/ml (120 μg). (H) Linear correlation of UV absorbance versus EGFP concentration was calculated by linear regression. The correlation of coefficient (R²) is 0.9988, as shown in the graph.