Role of PGM3 in sulforphane-induced apoptosis in LNCaP cells. (A) Effect of SFN on the expression of PGM3 protein in LNCaP cells treated with SFN (0, 20, 40 or 80 μM), examined by Western blotting. (B and C) Cells were transiently transfected with control or PGM3 siRNA. PGM3 expression was analyzed at protein and mRNA levels by Western blotting (B) and RT-PCR (C), respectively. (D) Effect of PGM3 siRNA on cytotoxic morphological changes in LNCaP cells treated with or without SFN. (E) TUNEL assay in PGM3 transfected LNCaP cells with or without SFN (40 μM) under an Axio vision 4.0 fluorescence microscope (×630).