Skip to main content

Table 1 Identification and quantification of aconitase by dMS and SC.

From: A label-free differential quantitative mass spectrometry method for the characterization and identification of protein changes during citrus fruit development

dMS

  

Stage II vs. early Stage II

Stage III vs. Stage II

      

HarvEST Citrus ID

iCitrus ID

Blast Hit to TAIR ID

Ratio

Pvalue

No. peptides

Max. Xcorr

Ratio

Pvalue

No. peptides

Max. Xcorr

      

UC46_7402

39802

AT2G05710

24.9

9.9E-20

3

5.39

33.6

3.621E-07

4

4.56

      

UC46_5405

43680

AT2G05710

25.3

9.9E-20

2

5.39

13.6

9.9E-20

2

4.56

      

UC46_5554

47264

AT2G05710

62.0

9.9E-20

2

5.40

4.9

0.0007414

3

6.52

      

UC46_5555

45840

AT2G05710

49.7

9.9E-20

4

5.40

4.8

0.0014824

4

6.52

      

UC46_9228

55395

AT2G05710

25.3

9.9E-20

2

5.39

9.4

4.115E-06

3

5.07

      

Spectral Counting

Stage II vs. early Stage II

Stage III vs. Stage II

HarvEST Citrus ID

iCitrus ID

Blast Hit to TAIR ID

Bayes

Factor

Fold

Change

Direction

No. peptides

-Log(e)

FDR Down

FDR

up

Bayes Factor

Fold

Change

Direction

No. peptides

-Log(e)

FDR Down

FDR up

UC46_7402

39802

AT2G05710

0.66

1.88

0

4

9.3

1.26

0.91

30.46

5.52

1

3

4.6

0.80

0.00

UC46_5405

43680

AT2G05710

-

-

-

-

-

-

-

143.42

12.24

1

2

3.7

1.03

0.88

UC46_5555

45840

AT2G05710

0.4

1.08

0

3

14.9

1.20

0.91

0.58

1.29

0

4

13.0

0.89

0.75

UC46_9228

55395

AT2G05710

0.42

1.08

0

2

11.7

1.26

0.91

0.68

1.11

0

3

11.0

0.73

0.86

 

HarvEST

 

UC46_7402

UC46_5405

UC46_5555

UC46_5554

UC46_9228

         

Origin

Citrus ID

iCitrus ID

39802

43680

45840

47264

55395

         

C. Sinensis

UC46_7402

39802

 

87

17

18

86

         

C. Sinensis

UC46_5405

43680

  

87

86

86

         

C. Sinensis

UC46_5555

45840

   

99

90

         

P. Trifoliata

UC46_5554

47264

    

89

         

C. Sinensis

UC46_9228

55395

              
  1. All iCitrus accessions for aconitase that were identified by both methods were homolog to the Arabidopsis gene At2g05710. Identification of aconitase by dMS and SC. The column "direction" under SC represents up-regulated = 1, no change = 0, down-regulated = -1. Aconitase iCitrus accessions amino acids sequences similarities.