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Table 1 Identification and quantification of aconitase by dMS and SC.

From: A label-free differential quantitative mass spectrometry method for the characterization and identification of protein changes during citrus fruit development

dMS    Stage II vs. early Stage II Stage III vs. Stage II       
HarvEST Citrus ID iCitrus ID Blast Hit to TAIR ID Ratio Pvalue No. peptides Max. Xcorr Ratio Pvalue No. peptides Max. Xcorr       
UC46_7402 39802 AT2G05710 24.9 9.9E-20 3 5.39 33.6 3.621E-07 4 4.56       
UC46_5405 43680 AT2G05710 25.3 9.9E-20 2 5.39 13.6 9.9E-20 2 4.56       
UC46_5554 47264 AT2G05710 62.0 9.9E-20 2 5.40 4.9 0.0007414 3 6.52       
UC46_5555 45840 AT2G05710 49.7 9.9E-20 4 5.40 4.8 0.0014824 4 6.52       
UC46_9228 55395 AT2G05710 25.3 9.9E-20 2 5.39 9.4 4.115E-06 3 5.07       
Spectral Counting Stage II vs. early Stage II Stage III vs. Stage II
HarvEST Citrus ID iCitrus ID Blast Hit to TAIR ID Bayes
Factor
Fold
Change
Direction No. peptides -Log(e) FDR Down FDR
up
Bayes Factor Fold
Change
Direction No. peptides -Log(e) FDR Down FDR up
UC46_7402 39802 AT2G05710 0.66 1.88 0 4 9.3 1.26 0.91 30.46 5.52 1 3 4.6 0.80 0.00
UC46_5405 43680 AT2G05710 - - - - - - - 143.42 12.24 1 2 3.7 1.03 0.88
UC46_5555 45840 AT2G05710 0.4 1.08 0 3 14.9 1.20 0.91 0.58 1.29 0 4 13.0 0.89 0.75
UC46_9228 55395 AT2G05710 0.42 1.08 0 2 11.7 1.26 0.91 0.68 1.11 0 3 11.0 0.73 0.86
  HarvEST   UC46_7402 UC46_5405 UC46_5555 UC46_5554 UC46_9228          
Origin Citrus ID iCitrus ID 39802 43680 45840 47264 55395          
C. Sinensis UC46_7402 39802   87 17 18 86          
C. Sinensis UC46_5405 43680    87 86 86          
C. Sinensis UC46_5555 45840     99 90          
P. Trifoliata UC46_5554 47264      89          
C. Sinensis UC46_9228 55395               
  1. All iCitrus accessions for aconitase that were identified by both methods were homolog to the Arabidopsis gene At2g05710. Identification of aconitase by dMS and SC. The column "direction" under SC represents up-regulated = 1, no change = 0, down-regulated = -1. Aconitase iCitrus accessions amino acids sequences similarities.