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Table 4 Structure-related proteins identified by dMS and SC.

From: A label-free differential quantitative mass spectrometry method for the characterization and identification of protein changes during citrus fruit development

    Stage II (55 mm) vs. early stage II (35 mm) Stage III (80 mm) vs. Stage II (55 mm)
    dMS SC dMS SC
Annotation iCitrus ID Blast Hit TAIR ID Peptides Ratio Bayes Factor Fold Change Direction* Peptides Ratio Bayes Factor Fold Change Direction*
TUB1 (tubulin beta-1) 33157 AT1G75780 4 0.024 -- -- -- 2 0.06 -- -- --
TUA3 (tubulin alpha-3) 46363 AT5G19770 4 0.07 -- -- -- -- -- -- -- --
TUA4 (tubulin alpha-4) 22742 AT1G04820 10 0.025 24.68 2.83 -1    1473619 13.49 -1
TUA4 (tubulin alpha-4) 28975 AT1G04820 10 0.025 -- -- -- 4 0.16 -- -- --
TUA4 (tubulin alpha-4) 37760 AT1G04820 8 0.026 884.12 10.19 -1 3 0.7 0.6 1.4 0
TUB5 (tubulin beta-5) 30386 AT1G20010 9 0.02 17.65 4.6 -1 4 0.41 527 9.15 -1
TUB5 (tubulin beta-5) 32879 AT1G20010 9 0.04 3125 46.15 -1 -- -- -- -- --
TUB5 (tubulin beta-5) 50323 AT1G20010 10 0.02 27.89 14.3 -1 3 0.4 16293 33.34 -1
TUB5 (tubulin beta-5) 51971 AT1G20010 10 0.025 537 26.95 -1 4 0.39 12048 32.23 -1
TUB5 (tubulin beta-5) 53023 AT1G20010 9 0.033 3784 49.2 -1 -- -- -- -- --
TUA6 (tubulin alpha-6) 2232 AT4G14960 7 0.044 -- -- -- -- -- -- -- --
TUA6 (tubulin alpha-6) 48045 AT4G14960 4 0.025 -- -- -- 2 21.7 -- -- --
TUB6 (tubulin beta-6) 54455 AT5G12250 6 0.027 -- -- --   2.18 -- -- --
TUB7 (tubulin beta-7) 54189 AT2G29550 2 0.6 -- -- -- 2 2.19 -- -- --
TUB8 (tubulin beta-8) 55174 AT5G23860 5 0.022 -- -- -- 3 45.85 -- -- --
ACT1 (actin 1) 45172 AT2G37620 3 0.3 -- -- -- 3 22.49 -- -- --
ACT7 (actin 7) 3401 AT5G09810 6 0.025 42.61 2.24 -1 -- -- -- -- --
ACT7 (actin 7) 31765 AT5G09810 6 0.22 -- -- -- 5 17.50 -- -- --
ACT8 (actin 8) 57479 AT1G49240 13 0.04 20.5 2.12 -1 8 21.93 20.36 2.12 1
ACT11 (actin 11) 51444 AT3G12110 4 0.26 -- -- -- 3 27.16 -- -- --
CAM5 (Calmodulin 5) 18478 AT2G27030 -- -- 7135 1.85 -1 -- -- 32767 2.69 -1
CAM5 (Calmodulin 5) 51416 AT2G27030 7 0.15 -- -- -- -- -- -- -- --
KIS (KIESEL) 26013 AT2G30410 -- -- 51.78 4.89 -1 -- -- 48.8 8.36 -1
VLN3 (VILLIN 3) 11096 AT3G57410 -- -- 1.19 3.00 0 -- -- 1.55 1.74 0
microtubule associated protein (MAP65/ASE1) 38803 AT4G26760 4 0.03 95.54 28.3 -1 -- -- 135.6 13.6 -1
PFN1/PRF1 (PROFILIN 1) 4145 AT2G19760 2 1.36 -- -- -- 2 15.81 0.80 1.14 0
PFN3/PRF3 (PROFILIN 3) 23065 AT5G56600 3 1.47 0.85 1.4 0 2 15.81 1.4 3.8 0
PRF5 (PROFILIN5) 5851 AT2G19770 -- -- 0.9 1.34 0 -- -- 0.6 1.03 0
ADF4 actin depolymerizing 7407 AT5G59890 -- -- 6.8 5.2 0 -- -- 28.89 10.4 -1
  1. * The column "direction" under spectral counting measurement represent expression direction, 1 = up-regulated, 0 = no change, -1 = down-regulated.
  2. Proteins identified by dMS were considered to be up-regulated when expression fold > 2, not changed when 0.5 < fold < 2 and down-regulated when fold change was < 2. For spectral counting Bayes factor of > 10 was used for significance difference.