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Table 5 Transport-related proteins identified by dMS and SC.

From: A label-free differential quantitative mass spectrometry method for the characterization and identification of protein changes during citrus fruit development

   

Stage II (55 mm) vs. early stage II (35 mm)

Stage III (80 mm) vs. Stage II (55 mm)

   

dMS

SC

dMS

SC

Annotation

iCitrus ID

Blast Hit TAIR ID

Peptides

Ratio

Bayes Factor

Fold Change

Direction*

Peptides

Ratio

Bayes Factor

Fold Change

Direction*

Ca2+ -ATPase

45644

AT1G07670

--

--

1.2

2.9

0

2

20.88

27.99

4.99

1

Ca2+/Na+ exchanger

47787

AT1G53210

2

0.27

14.27

3.00

-1

2

9.04

0.71

1.15

0

KEA2 (K+/H+ antiporter)

36862

AT4G00630

2

0.40

1.41

1.75

0

--

--

0.8

1.2

0

porin, putative; voltage-dependent anion channel

23759

AT3G01280

2

0.19

13.9

6.34

-1

2

6.09

15.91

4.81

1

DET3 C subunit C (V-type H+- ATPase)

58235

AT1G12840

3

0.27

45.53

3.26

-1

--

--

1.2

1.48

0

AVP1 (vacuolar H+-PPiase)

36100

AT1G15690

4

2.31

1.1

1.2

0

  

2.76

1.63

0

VHA-A

27256

AT1G78900

13

0.04

1011648

3.99

-1

12

9.94

38.82

2.2

1

VHA-A3 (V-ATPase)

49974

AT4G39080

4

0.44

--

--

--

3

4.01

--

--

--

V-type ATPase subunit B1

3683

AT1G76030

8

0.05

--

--

--

5

13.78

--

--

--

V-type ATPase subunit B2

62399

AT4G38510

11

0.06

--

--

--

9

11.25

--

--

--

V-type ATPase subunit B3

37671

AT1G20260

11

0.06

140.60

2.13

-1

9

11.25

1004

2.64

1

V-type ATPase subunit D

23422

AT3G58730

--

--

34.4

5.4

-1

--

--

1.55

1.45

0

V-type ATPase subunit E1

52391

AT4G11150

--

--

19.83

3.22

-1

2

5.12

7.23

1.94

0

VMA10 (V-type ATPase 10); Subunit G

28722

AT3G01390

3

0.28

36.6

2.8

-1

--

--

0.15

1.78

0

V-type ATPase subunit H

38700

AT3G42050

6

0.25

36.6

2.82

-1

4

13.57

0.58

1.06

0

AHA2 (H+-ATPase 2)

50370

AT4G30190

3

0.53

0.98

1.23

0

4

11.79

1.75

1.49

0

AHA4 (H+- ATPase 4)

28397

AT3G47950

3

1.14

11055

20.4

-1

3

7.40

0.9

1.5

0

AHA8 (H+- ATPase 8)

56228

AT3G42640

2

0.35

--

--

--

2

13.60

--

--

--

AHA10 (Autoinhibited H+- ATPase isoform 10)

56764

AT1G17260

4

1.13

5.8

1.55

0

4

41.48

1.36

1.19

0

H+- ATPase

42025

AT3G28710

2

0.11

335

18.72

-1

2

17.27

22.04

4.37

1

TMT1 (tonoplast monosaccharide transporter1)

44853

AT1G20840

3

24.79

3.0

1.1

0

--

--

382

5.1

1

TMT2, (tonoplast monosaccharide transporter 2

41259

AT4G35300

8

32.69

16121

2.64

1

6

15.26

9.5

1.9

0

GPT2 (glucose-6-phosphate/phosphate translocator 2)

44184

AT1G61800

2

0.10

28.2

12.3

-1

--

--

0.8

1.2

0

mannitol transporter

32978

AT2G16130

--

--

439

18.8

-1

--

--

5.8

3.2

0

LPT(lipid transfer protein)

45077

AT1G27950

2

0.01

5258.00

22.44

-1

--

--

--

--

--

LTP3

58907

AT5G59320

--

--

2.86

4

0

--

--

7447

21.8

-1

PDR6/PDR6 (pleiotropic drug resistance 6); ATPase

26072

AT2G36380

--

--

4.5

7.11

0

--

--

49.8

3.9

1

PDR11/PDR11 (pleiotropic drug resistance 11); ATPase

52044

AT1G66950

--

--

--

--

--

2

11.74

1.18

1.56

0

PDR12/(PLEIOTROPIC DRUG 12); ATPase

19314

AT1G15520

--

--

0.98

1.03

0

2

14.97

5.44

3.46

0

MAPR2 (membrane-associated progesterone binding protein 2)

28962

AT2G24940

3

1.62

1.57

1.65

0

3

45.68

1.38

1.69

0

MRP4 (multidrug resistance-associated protein 4)

46730

AT2G47800

2

1.36

2.62

2.64

0

5

58.12

19.3

2.6

1

PGP7 (P-GLYCOPROTEIN 7); ATPase

10552

AT5G46540

--

--

2.06

5.56

0

--

--

10.4

3.6

1

PIP1B (plasma membrane intrinsic protein 1;2); water transport

18285

AT2G45960

--

--

149.18

27.06

-1

--

--

--

--

--

PIP2;8/PIP3B (plasma membrane intrinsic protein 2;8); water channel

25444

AT2G16850

--

--

462

23

-1

--

--

0.9

1

0

PIP2;5/PIP2 D (plasma membrane intrinsic protein 2;5); water channel

41682

AT3G54820

--

--

635.26

39.02

-1

--

--

--

--

--

TMP-C (plasma membrane intrinsic protein 1;4); water channel

28678

AT4G00430

3

0.11

35.06

10.97

-1

--

--

2.76

2.70

0

protein transporter

51128

AT2G20760

3

30

0.2

1.6

0

2

1.80

0.84

1.22

0

protein transporter

21278

AT3G51890

2

118.94

1510

6.2

1

--

--

1.63

1.57

0

TOM20-3 (translocase of outer membrane)

33905

AT3G27080

4

0.45

0.6

1.16

0

3

4.75

18.02

2.97

1

ACP4 (acyl carrier 4)

25090

AT4G25050

3

0.34

3.1

2.27

0

--

--

13.4

7.8

1

AAC2 (ADP/ATP carrier)

53189

AT5G13490

8

0.27

16767

10.36

-1

5

21.36

1.01

1.02

0

AAC3 (ADP/ATP carrier)

26451

AT4G28390

10

0.22

1040

3.48

-1

6

76.16

32.95

2.75

1

mitochondrial phosphate transporter

44051

AT5G14040

4

0.10

10.8

3

-1

2

4.29

0.79

1.2

0

PDE120 protein import (Tic40)

57128

AT5G16620

2

0.34

--

--

--

--

--

--

--

--

di/tricarboxylate carrier

39221

AT5G19760

5

56.47

0.52

1.49

0

2

25.92

0.69

1.13

0

TOM22-V (translocase outer mitochondrial membrane)

38717

AT5G43970

--

--

160

17.8

-1

--

--

2.98

2.65

0

Lipocalin

23197

AT5G58070

2

29.74

44.2

4.3

1

3

10.49

40.34

2.3

1

  1. * The column "direction" under spectral counting measurement represent expression direction, 1 = up-regulated, 0 = no change, -1 = down-regulated.
  2. Proteins identified by dMS were considered to be up-regulated when expression fold > 2, not changed when 0.5 < fold < 2 and down-regulated when fold change was < 2. For spectral counting Bayes factor of > 10 was used for significance difference.