Figure 2From: RNAi-based validation of antibodies for reverse phase protein arrays Knockdown detection capacity of anti-EGFR antibodies. EGFR levels of siALLSTAR and siEGFR transfected MDA-MB-231 cells were quantified using two anti-EGFR antibodies and a linear regression model fitted on a serially diluted MDA-MB-231 cell lysate. Both antibodies showed identical readout for Western Blot-based knockdown quantification.Back to article page