Figure 5
From: RNAi-based validation of antibodies for reverse phase protein arrays
Target specificity and signal/concentration ratio of STAT3 antibodies. Ten antibodies targeting STAT3 were tested on lysates of MDA-MB-231 cells by Western blot. Antibodies 1-6 showed high specificity whereas antibodies 7-10 detected additional bands of lower molecular weight (A). RPPA signal dynamics of STAT3 antibodies probed on lysates of MDA-MB-231 cells (B).