Figure 6
From: RNAi-based validation of antibodies for reverse phase protein arrays
Comparing antibody signal dynamics and quantitative readout after STAT3 silencing. The RPPA signal range of six highly specific anti-STAT3 antibodies was correlated with the quantitative readout obtained for each of the respective antibodies when applied for the RPPA analysis of STAT3-silencing (R2). The data set was calculated twice by using each of the two independent calibrator dilution series (black and grey). The correlation coefficient was calculated using MS Excel, the linear regression line was added.