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Table 1 EGFR knockdown efficiency determined by RPPA

From: RNAi-based validation of antibodies for reverse phase protein arrays

Antibodya

Calibratorb

Arrayc

  

1

2

3

EGFR #1

1

59%

60%

61%

 

2

58%

58%

60%

EGFR #2

1

85%

85%

84%

 

2

85%

85%

84%

  1. a Two different anti-EGFR antibodies (#1, #2) were used to quantify the EGFR knockdown.
  2. b Each subarray contained two different calibrator dilution series (1, 2) printed in parallel with biological replicates of the EGFR knockdown and controls.
  3. c Three different subarrays (1-3) were printed and analyzed. Quantitative readout calculated in percent based on signals from control transfections with non-targeting siRNAs.