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Table 1 EGFR knockdown efficiency determined by RPPA

From: RNAi-based validation of antibodies for reverse phase protein arrays

Antibodya Calibratorb Arrayc
   1 2 3
EGFR #1 1 59% 60% 61%
  2 58% 58% 60%
EGFR #2 1 85% 85% 84%
  2 85% 85% 84%
  1. a Two different anti-EGFR antibodies (#1, #2) were used to quantify the EGFR knockdown.
  2. b Each subarray contained two different calibrator dilution series (1, 2) printed in parallel with biological replicates of the EGFR knockdown and controls.
  3. c Three different subarrays (1-3) were printed and analyzed. Quantitative readout calculated in percent based on signals from control transfections with non-targeting siRNAs.