Antibodya
| Calibratorb
| Arrayc
|
---|
| |
1
|
2
|
3
|
EGFR #1
| 1 | 59% | 60% | 61% |
| 2 | 58% | 58% | 60% |
EGFR #2
| 1 | 85% | 85% | 84% |
| 2 | 85% | 85% | 84% |
-
a Two different anti-EGFR antibodies (#1, #2) were used to quantify the EGFR knockdown.
-
b Each subarray contained two different calibrator dilution series (1, 2) printed in parallel with biological replicates of the EGFR knockdown and controls.
-
c Three different subarrays (1-3) were printed and analyzed. Quantitative readout calculated in percent based on signals from control transfections with non-targeting siRNAs.