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Table 1 EGFR knockdown efficiency determined by RPPA
RNAi-based validation of antibodies for reverse phase protein arrays
Two different anti-EGFR antibodies (#1, #2) were used to quantify the EGFR knockdown.
Each subarray contained two different calibrator dilution series (1, 2) printed in parallel with biological replicates of the EGFR knockdown and controls.
Three different subarrays (1-3) were printed and analyzed. Quantitative readout calculated in percent based on signals from control transfections with non-targeting siRNAs.
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