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Table 2 Quantification of STAT3 knockdown and determination of STAT3 detection limits

From: RNAi-based validation of antibodies for reverse phase protein arrays

Antibody Knockdown efficiency LOD [μg/μl]b
  1 a 2 a 1 a 2 a
AB1 75% 74% 0.027 0.033
AB2 82% 81% 0.035 0.038
AB3 86% 85% 0.057 0.057
AB4 84% 83% 0.016 0.018
AB5 69% 68% 0.029 0.031
AB6 -2% -3% 0.173 0.134
AB7 80% 80% 0.031 0.030
AB8 32% 33% 0.073 0.057
AB9 57% 56% 0.077 0.077
AB10 53% 52% 0.023 0.023
  1. a Two different MDA-MB231 dilution series (1, 2) were used to calculate the percentage of the STAT3 knockdown.
  2. b The limit of detection (LOD) was calculated for AB1-10 and is expressed as μg/μl MDA-MB231 lysate.