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Table 2 Quantification of STAT3 knockdown and determination of STAT3 detection limits

From: RNAi-based validation of antibodies for reverse phase protein arrays

Antibody

Knockdown efficiency

LOD [μg/μl]b

 

1 a

2 a

1 a

2 a

AB1

75%

74%

0.027

0.033

AB2

82%

81%

0.035

0.038

AB3

86%

85%

0.057

0.057

AB4

84%

83%

0.016

0.018

AB5

69%

68%

0.029

0.031

AB6

-2%

-3%

0.173

0.134

AB7

80%

80%

0.031

0.030

AB8

32%

33%

0.073

0.057

AB9

57%

56%

0.077

0.077

AB10

53%

52%

0.023

0.023

  1. a Two different MDA-MB231 dilution series (1, 2) were used to calculate the percentage of the STAT3 knockdown.
  2. b The limit of detection (LOD) was calculated for AB1-10 and is expressed as μg/μl MDA-MB231 lysate.