Table 1 Comparative analysis of proteins regulated in a control culture medium or in a stress medium inducing flocculosin synthesis in Pseudozyma flocculosa cells.
Spot | Protein name | GenBank accession number | Mascot Score | Organism homology and respective GenBank accession number | Peptides sequences (LC MS/MS) |
|---|---|---|---|---|---|
Proteins specific to the stress treatment | |||||
F33 | Thiamine biosynthesis | GQ922830 | 258 | Ustilago maydis 71004594 | K.VALLEPNDPSDVTEIIGSGK.V (2+) K.IGYVGEFGK.I (2+) |
F5 | Glutamate synthase | GQ922824 | 255 | Ustilago maydis 71019533 | K.GKDVIVIGGGDTGCDAQATALR.H (3+) R.LLMTNNFPEFTGR.V (2+) R.EADGVHFAMDFLAPNTK.S (3+) R.EYCISTTSFEK.D (2+) R.IGGLLMYGIPNMK.L (2+) |
F8 | Aspartic acid protease | 157418069 | 96 | Pseudozyma flocculosa 157418069 | K.GTVYTDALTIGGLTASR.V (2+) K.LNAGVFQFTLAK.T (2+) |
F11 | 24 kDa RAS-like protein | GQ922825 | 78 | Ustilago maydis 71004934 | R.NSFDEISTFHQQILR.V (3+) R.INVDEAFSNLVR.E (2+) |
Proteins up-regulated in the stress treatment | |||||
F23 | Cyanate lyase | GQ922826 | 111 | Ustilago maydis 71007509 | R.LYEVLVVYGYPLK.H (2+) R.DPVLYR.L (2+) |
F46 | Transketolase | GQ922827 | 84 | Ustilago maydis 39968757 | K.IEAAIPELVGGSADLTGSNLTR.W (3+) |
F24 | Electron transfer flavoprotein beta subunit | GQ922828 | 201 | Ustilago maydis 71004000 | K.FSMNPFDEIAVEEAVR.L (2+) |
F42 | Triosephosphate isomerase | GQ922829 | 75 | Ustilago maydis 164661864 | K.VATAEQAQEVHAAIR.Q (3+) |
F39 | Transaldolase | GQ922831 | 268 | Ustilago maydis 71020109 | K.IASTWEGIQAAR.E (2+) K.LAEGIAGFAK.D (2+) K.TIVMGASFR.N (2+) |
F40 | Heat shock protein of HSP70 family | GQ922832 | 286 | Ustilago maydis 71020701 | R.LLGEFELTGIPPQPR.G (3+) R.VFTTVEDNQTQVTFPVYEGER.T (3+) R.KANITITNSVGR.L (3+) K.ANITITNSVGR.L (2+) |
F27 | Heat-shock protein 90* | GQ922823 | 177 | Cryptococcus neoformans 25990448 | K.GIVDSEDLPLNISR.E (2+) R.ELISNASDALDK.I (2+) |
F37 | ATP synthase beta chain | GQ922833 | 905 | Ustilago maydis 71018215 | R.IMNVIGEPMDER.G (2+) R.FTQAGSETSALLGR.I (3+) K.TVLIQELINNVAK.A(2+) K.VALVFGQMNEPPGAR.A(3+) |
F25 | Septin-8 | GQ922834 | 180 | Ustilago maydis 71019031 | K.LTVIDTPGFGDYVNNR.D (2+) K.AGGHFTLMVVGESGVGK.T (3+) K.TVEIDIIK.A (2+) |
F26 | Succinate dehydrogenase [ubiquinone] flavoprotein subunit | GQ922835 | 232 | Ustilago maydis 71005306 | R.LGANSLLDIVVFGR.A (2+) R.TVIELEHFGLPFSR.T (3+) R.VMQSDAAVFR.T (2+) |
F43 | Alpha glucosidase precursor | GQ922836 | 116 | Ustilago maydis 71016306 | R.RDPDETLQPFFTLDAGTPVDSNMYGYHPVYTEAR.R (3+) R.RGLIQYR.A (2+) |
Proteins absent in the stress treatment | |||||
F14 | Elongation factor 1-alpha | GQ922837 | 304 | Ustilago maydis 71004810 | K.IGGIGTVPVGR.V (2+) K.STTTGHLIYK.C (2+) R.EHALLAFTLGVR.Q 2(+) K.YYVTVIDAPGHR.D 3(+) K.SVEMHHEQLPEGLPGDNVGFNVK.N (3+) |
F17 | Ornithine amino-transferase | GQ922838 | 132 | Ustilago maydis 71018171 | K.GLLAKPTHVNIIR.L (3+) R.TGFGPFLDR.V (2+) |
F18 | 6-phospho-gluconate dehydrogenase | GQ922839 | 99 | Ustilago maydis 71014537 | K.GILFVGSGVSGGEEGAR.H (2+) K.IVSYAQGFMLMR.E (2+) |
Proteins down-regulated in the stress treatment | |||||
F28 | Phospho-glycerate kinase | GQ922840 | 280 | Ustilago maydis 71021575 | K.IQLIDNMLDK.V (2+) K.YSLKPVAAEVSK.L (2+) K.VNSLIICGGMAFTFK.K (3+) K.ALESPERPFLAILGGAK.V (3+) |
F29 | Phospho-glycerate kinase | GQ922840 | 62 | Ustilago maydis 71021575 | K.ALESPERPFLAILGGAK.V (2+) K.VNSLIICGGMAFTFK.K (2+) K.IQLIDNMLDK.V (2+) |
F30 | Glucose-6-phosphate 1-dehydrogenase | GQ922841 | 293 | Ustilago maydis 71021693 | K.LVDNVQITFK.E (2+) K.SFSAEDIRDEK.V (2+) K.DVTSGIFKDIPR.N (2+) |