Figure 4
From: A proteomic analysis of C-reactive protein stimulated THP-1 monocytes
Quantitative analysis of differentially expressed proteins. A. mCRP induces expression of cellular stress related proteins of the ubiquitin/proteasome system and the heat shock response.
Expression levels of several proteins of interest were quantified and are given in the graphs as mean +/- standard deviation. # indicates p < 0.05 compared to control. mCRP up-regulates UBE1, and HSP 70, as well as the alpha isoform of HSP 105. pCRP induces the beta isoform of HSP105.
B. Differential expression of proteins involved in the inflammatory response. ACTN4 and RIPK2 are increased in the LPS, as well as in the m- and pCRP group. ENOA is increased by LPS and mCRP stimulation of THP-1 monocytes, whereas EHD1 is significantly upregulated after stimulation with LPS.
C. Western blot analysis of selected proteins that are differentially expressed in THP-1 cells. Selected Proteins were validated by Western blotting. Western blot results are shown exhibiting specific staining of 1D-gels with anti-HSP70, anti-RIPK2 and anti-UBE1 antibodies. Analysis served as a confirmation of the protein identity in addition to the Mass-spec analysis.