Figure 6
From: A proteomic analysis of C-reactive protein stimulated THP-1 monocytes
Incubation of pCRP in polystyrene flasks leads to a partial dissociation of the protein to mCRP. After various hours of incubation time samples of the incubation assays with pCRP were dot-blotted and the blots probed with mCRP (clone 9C9) or pCRP (clone 8D8) specific antibodies. Whereas the pCRP staining shows a mild decrease the mCRP staining increases gradually in the course of the experiment.