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Table 10 Identification and quantification of enzymes and other factors by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot # Predominant protein1 Total
proteins
in spot2
Spot
Volume3
Std
Dev4
MS/MS
Coverage5
Predicted
MW6
pI7
  Beta-amylase (β-Amy-A1, β-Amy-B1, β-Amy-D1)       
476 Beta-amylase Bu-18 4 - - 32 60016 6.9
537 Beta-amylase Bu-1 2 0.04 0.010 18 60016 6.9
94 Beta-amylase Bu2 2 0.04 0.006 47 54481 5.9
462 Beta-amylase Bu2 2 0.11 0.014 60 54481 5.9
64 Beta-amylase Bu3 1 0.05 0.010 13 54319 5.8
92 Beta-amylase Bu3 1 0.18 0.029 34 54319 5.8
93 Beta-amylase Bu3 1 0.09 0.013 55 54319 5.8
  Other enzymes 9       
89 ADP-glucose PP lg subunit [GenBank:CAD98749] 1 0.03 0.002 10 53030 5.6
128 ADP-glucose PP lg subunit [GenBank:CAD98749] 1 0.10 0.047 11 53030 5.6
108 ADP-glucose PP sm subunit [GenBank:AAF61173] 2 0.09 0.013 60 52061 5.5
110 ADP-glucose PP sm subunit [GenBank:AAF61173] 2 0.06 0.019 24 52061 5.5
118 Alanine amino transferase TC11_282456 1 0.06 0.002 36 52820 6.1
109 ATP-synthase beta-subunit [GenBank:CAA52636] 1 0.02 0.004 32 58562 5.4
232 Chitinase, rye, [GenBank:BAB18520] 1 0.04 0.009 40 26095 8.7
455 Chitinase [GenBank:AAX83262] 1 0.05 0.010 26 26022 8.3
241 Dehydroascorbate reductase TC264934 2 0.09 0.013 83 23358 5.9
123 Enolase TC11_292359 2 0.07 0.014 64 48033 5.4
178 Glyoxalase I TC11_288238 1 0.10 0.020 68 32568 5.4
202 Glucose/ribitol dehydrogenase RS_UWI_14903 1 <0.01 0.000 57 31851 6.3
436 Ketol-acid reducto isomerase TC234371 3 0.05 0.005 21 57486 5.4
175 Malate dehydrogenase [GenBank:AAT64932] 1 0.03 0.007 41 35486 5.8
176 Malate dehydrogenase [GenBank:AAT64932] 1 0.15 0.000 58 35486 5.8
450 Methionine synthase RS_UWI_10957 1 0.02 0.005 11 84552 5.7
371 Orthophosphate dikinase TC11_322894 1 0.03 0.004 19 73501 5.8
299 PDI3 [GenBank:AAK49425] 3 0.14 0.016 53 54094 4.9
475 Sucrose synthase 2 [GenBank:CAA03935] 1 0.08 0.011 19 92608 6.2
189 Thiamine biosynth enzyme TC11_308909 2 0.08 0.007 35 33167 5.7
225 Triose-phosphate isomerase [GenBank:CAC14917] 1 0.05 0.006 38 26803 5.4
239 27 K thiol reductase-like TC11_300123 1 0.16 0.037 54 23642 6.1
479 27 K thiol reductase like TC11_299048 2 0.02 0.005 16 23788 6.1
  Other       
311 Elongation factor EF1A [Swiss-Prot: Q03033] 1 0.11 0.005 14 49169 9.2
533 HSP70 (Butte 86) [GenBank:AAB99745] 1 0.08 0.009 18 71031 5.1
413 Initiation factor Eif4A [Swiss-Prot: P41378.1] 1 0.06 0.014 36 46928 5.3
295 LTP Bu-2 1 0.04 0.009 38 9606 8.2
456 Thaumatin-like protein TC11_283136 2 0.05 0.007 39 21408 7.9
  1. Loci are indicated within parentheses if known.
  2. 1 Protein that matched the greatest number of peptides detected in spot by MS/MS. Accession numbers from TaGI Release 10.0 are denoted with the prefix TC and TaGI release 11.0 with the prefix TC11_. Accession numbers from HarvEST 1.14 are denoted with RS_UWI_. Sequences of proteins from Butte 86 are found in Additional file 2.
  3. 2-7 Footnotes as in Table 3.
  4. 8Although Scaffold identified more peptides for beta-amylase in spot 476, other data indicate that this spot was predominantly omega-gliadin.
  5. 9The following enzymes or factors were detected as minor components of other spots: alcohol dehydrogenase, aspartic protease, formate dehydrogenase, fructose bisphosphate aldolase, GDP-binding protein, manganese superoxide dismutase, peroxidase, peroxiredoxin, phosphoglycerate kinase.