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Table 2 2DE-MS/MS identification of wheat flour proteins.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

   Scaffold Assignments1 Final Assignments2
Protein type Total
Spots 3
Total
Sequences
NCBI
nr
Contig
databases 4
Butte 86
Contigs
or ESTs 5
Final
Sequences
Butte 86
Contigs or
ESTs 5
HMW-GS 43 6 4 2 0 5 0
LMW-GS 35 24 9 4 11 22 10
Gamma-gliadins 34 13 2 1 10 13 10
Omega-gliadins 13 8 2 2 4 7 4
Alpha-gliadins 33 34 8 16 10 23 16
Farinins 11 3 0 0 3 3 3
Purinins 6 3 0 0 3 3 3
Triticins 9 3 0 3 0 3 0
Globulins 11 8 1 4 3 8 3
GSPs/Puroindolines 4 3 3 0 0 3 0
Alpha-amylase inhibitors 21 16 9 7 0 16 0
Serpins 15 7 2 5 0 9 6
Beta-amylases 12 4 0 1 3 4 3
Other enzymes6 42 30 12 18 0 33 0
Other proteins 15 6 3 2 1 5 1
Total   168 55 65 48 157 59
  1. Numbers of spots and protein sequences and their database origins for each major protein category.
  2. 1Scaffold assignments for each spot are in Additional files 4, 5, 6, 7, 8, 9.
  3. 2Final assignments for each spot are in Additional file 1.
  4. 3Includes all 2-DE spots in which two or more peptides corresponded to sequences for the indicated protein type.
  5. 4Includes TaGI Releases 10.0 and 11.0, US Wheat Genome Project Assembly, HarvEST 1.14 and NCBI Unigene Build #55.
  6. 5Sequences derived from Butte 86 contigs are in Additional files 2, 3. Butte 86 sequences for some protein types were not analyzed in this study.
  7. 6Includes sequences for nine enzymes that were minor components of other spots as detailed in Additional file 1.