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Table 3 Identification and quantification of HMW-GS by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot # Predominant protein1 Total
proteins2
Spot
Volume3
Std
Dev4
MS/MS
Coverage5
Predicted
MW6
pI7
  HMW-GS Ax2* (Glu-A1b-1)       
12 HMW-GS Ax2* [GenBank:AAB02788] 1 1.22 0.024 52 86336 5.7
16 HMW-GS Ax2* [GenBank:AAB02788] 2 0.07 0.004 13 86336 5.7
17 HMW-GS Ax2* [GenBank:AAB02788] 1 0.36 0.019 34 86336 5.7
18 HMW-GS Ax2* [GenBank:AAB02788] 1 0.05 0.001 7 86336 5.7
19 HMW-GS Ax2* [GenBank:AAB02788] 1 0.14 0.005 10 86336 5.7
23 HMW-GS Ax2* [GenBank:AAB02788] 1 0.40 0.006 26 86336 5.7
24 HMW-GS Ax2* [GenBank:AAB02788] 1 0.13 0.006 22 86336 5.7
28 HMW-GS Ax2* [GenBank:AAB02788] 1 0.03 0.004 8 86336 5.7
   Total 2.40     
  HMW-GS Bx7 (Glu-B1c-1)       
20 HMW-GS Bx7 [GenBank:CAA32115] 1 1.79 0.053 56 82527 8.4
21 HMW-GS Bx7 [GenBank:CAA32115] 1 2.28 0.063 57 82527 8.4
25 HMW-GS Bx7 [GenBank:CAA32115] 1 0.12 0.026 6 82527 8.4
26 HMW-GS Bx7 [GenBank:CAA32115] 1 0.48 0.016 10 82527 8.4
27 HMW-GS Bx7 [GenBank:CAA32115] 1 0.18 0.004 5 82527 8.4
29 HMW-GS Bx7 [GenBank:CAA32115] 1 0.02 0.002 3 82527 8.4
33 HMW-GS Bx7 [GenBank:CAA32115] 1 0.05 0.002 3 82527 8.4
302 HMW-GS Bx7 [GenBank:CAA32115] 1 0.10 0.009 3 82527 8.4
   Total 5.02     
  HMW-GS By9 (Glu-B1c-2)       
40 HMW-GS By9 [GenBank:CAA43361] 2 0.38 0.003 34 73518 8.4
47 HMW-GS By9 [GenBank:CAA43361] 1 0.05 0.002 10 73518 8.4
52 HMW-GS By9 [GenBank:CAA43361] 1 0.05 0.004 19 73518 8.4
349 HMW-GS By9 [GenBank:CAA43361] 1 0.11 0.016 27 73518 8.4
350 HMW-GS By9 [GenBank:CAA43361] 1 1.32 0.004 56 73518 8.4
351 HMW-GS By9 [GenBank:CAA43361] 2 0.15 0.017 45 73518 8.4
358 HMW-GS By9 [GenBank:CAA43361] 1 0.08 0.007 20 73518 8.4
360 HMW-GS By9 [GenBank:CAA43361] 1 0.14 0.021 48 73518 8.4
   Total 2.28     
  HMW-GS Dx5 (Glu-D1d-1)       
3 HMW-GS Dx5 [GenBank:ABG68042] 1 2.37 0.020 50 87941 6.1
5 HMW-GS Dx5 [GenBank:ABG68042] 1 0.70 0.043 45 87941 6.1
7 HMW-GS Dx5 [GenBank:ABG68042] 1 0.16 0.007 3 87941 6.1
10 HMW-GS Dx5 [GenBank:ABG68042] 1 0.06 0.004 19 87941 6.1
14 HMW-GS Dx5 [GenBank:ABG68042] 1 0.05 0.007 16 87941 6.1
36 HMW-GS Dx5 [GenBank:ABG68042] 1 0.07 0.006 4 87941 6.1
138 HMW-GS Dx5 [GenBank:ABG68042] 1 0.21 0.024 4 87941 6.1
307 HMW-GS Dx5 [GenBank:ABG68042] 1 0.08 0.028 21 87941 6.1
538 HMW-GS Dx5 [GenBank:ABG68042] 1 0.05 0.002 3 87941 6.1
   Total 3.75     
  HMW-GS Dy10 (Glu-D1d-2)       
42 HMWGS Dy10 [Swiss-Prot:P10387] 2 2.32 0.051 57 67475 7.0
48 HMWGS Dy10 [Swiss-Prot:P10387] 1 0.21 0.032 57 67475 7.0
51 HMWGS Dy10 [Swiss-Prot:P10387] 2 0.17 0.014 57 67475 7.0
333 HMWGS Dy10 [Swiss-Prot:P10387] 1 0.65 0.030 57 67475 7.0
407 HMWGS Dy10 [Swiss-Prot:P10387] 1 0.07 0.019 57 67475 7.0
424 HMWGS Dy10 [Swiss-Prot:P10387] 1 0.20 0.014 57 67475 7.0
514 HMWGS Dy10 [Swiss-Prot:P10387] 1 0.07 0.010 57 67475 7.0
   Total 3.69     
  1. Loci are indicated within parentheses.
  2. 1Protein that matched the greatest number of peptides detected in spot by MS/MS.
  3. 2Identifications of all proteins within each spot are given in Additional file 1.
  4. 3Normalized spot volume, average of three determinations.
  5. 4Standard deviation for three determinations.
  6. 5Percentage coverage of the mature protein by identified peptides.
  7. 6Predicted size of the mature protein in Daltons detemined by ExPASy ProtParam.
  8. 7Predicted pI for the mature protein determined by ExPASy ProtParam.