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Table 4 Identification and quantification of LMW-GS by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot # Predominant protein1 Total
proteins
in spot2
Spot
Volume3
Std
Dev4
MS/MS
Coverage5
Predicted
MW6
pI7
  LMW-GS QISQQQ-type 8 (Glu-A3f)       
125 LMW-GS [GenBank:AAB48469] 2 0.32 0.181 41 39417 8.5
140 LMW-GS Bu4/[Swiss-Prot:P10385] 1 0.19 0.018 50 39130 8.9
141 LMW-GS Bu4/[Swiss-Prot:P10385] 1 1.10 0.031 51 39130 8.9
141a LMW-GS Bu4/[Swiss-Prot:P10385] 2 0.58 0.059 16 39130 8.9
   Total 2.19     
  LMW-GS QMENSHIP-type 8 (Glu-B3h)       
314 LMW-GS Bu-2/12/13 [GenBank:ABC84366] 2 1.35 0.163 44 38153 8.2
317 LMW-GS Bu-2/12/13 [GenBank:ABC84366] 3 0.35 0.017 45 38153 8.2
318 LMW-GS Bu-2/12/13 [GenBank:ABC84366] 3 0.34 0.046 33 38153 8.2
322 LMW-GS Bu-2/12/13 [GenBank:ABC84366] 2 0.37 0.008 28 38153 8.2
119 LMW-GS Bu-3/[GenBank:BAD12055] 1 2.40 0.354 79 42589 8.5
119a LMW-GS Bu-3/[GenBank:BAD12055] 2 0.69 0.256 62 42589 8.5
120 LMW-GS Bu-3/[GenBank:BAD12055] 1 0.78 0.112 61 42589 8.5
131 LMW-GS Bu-3/[GenBank:BAD12055] 1 0.72 0.047 48 42589 8.5
132 LMW-GS Bu-3/[GenBank:BAD12055] 1 0.10 0.008 39 42589 8.5
161 LMW-GS Bu-3/[GenBank:BAD12055] 3 <0.01 0.000 23 42589 8.5
237 LMW-GS Bu-3/[GenBank:BAD12055] 1 0.03 0.003 16 42589 8.5
310 LMW-GS Bu-3/[GenBank:BAD12055] 3 1.13 0.054 50 42589 8.5
316 LMW-GS Bu-3/[GenBank:BAD12055] 3 0.21 0.033 36 42589 8.5
   Total 8.47     
  LMW-GS QMET-type 8 (Glu-D3a)       
167 LMW-GS Bu-1 4 2.10 0.037 89 33008 8.7
170 LMW-GS Bu-1 3 0.33 0.021 66 33008 8.7
173 LMW-GS Bu-6 3 0.47 0.007 78 31901 8.9
144 LMW-GS Bu-7 1 1.52 0.040 50 37700 8.2
145 LMW-GS Bu-7 2 0.78 0.021 55 37700 8.2
472 LMW-GS Bu-7 3 0.17 0.071 48 37700 8.2
343 LMW-GS Bu-8 3 0.29 0.057 61 32175 7.7
203 LMW-GS Bu-11/[GenBank:AAT3786] 2 <0.01 0.000 11 39788 8.5
315 LMW-GS Bu-11/[GenBank:AAT3786] 2 0.27 0.027 52 39788 8.5
153 LMW-GS Bu-18/TC250064 1 0.26 0.030 49 37126 8.5
155 LMW-GS Bu-18/TC250064 2 0.47 0.012 50 37126 8.5
319 LMW-GS TC11_277270 2 0.67 0.038 55 39762 8.5
   Total 7.33     
  1. Loci are indicated within parentheses.
  2. 1Protein that matched the greatest number of peptides detected in spot by MS/MS. Accession numbers from TaGI Release 10.0 are denoted with the prefix TC and TaGI release 11.0 with the prefix TC11_. Sequences of proteins from Butte 86 are found in Additional file 2. For partial Butte 86 contigs, the accession number for the most similar complete sequence is also indicated.
  3. 2-7Footnotes as in Table 3.
  4. 8N-terminal sequences predicted by Target P.