Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Dupont, Frances M; Vensel, William H; Tanaka, Charlene K; Hurkman, William J; Altenbach, Susan B

doi:10.1186/1477-5956-9-10

Table 4 Identification and quantification of LMW-GS by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot #	Predominant protein¹	Total proteins in spot²	Spot Volume³	Std Dev⁴	MS/MS Coverage⁵	Predicted MW⁶	pI⁷
	LMW-GS QISQQQ-type ⁸ (Glu-A3f)
125	LMW-GS [GenBank:AAB48469]	2	0.32	0.181	41	39417	8.5
140	LMW-GS Bu4/[Swiss-Prot:P10385]	1	0.19	0.018	50	39130	8.9
141	LMW-GS Bu4/[Swiss-Prot:P10385]	1	1.10	0.031	51	39130	8.9
141a	LMW-GS Bu4/[Swiss-Prot:P10385]	2	0.58	0.059	16	39130	8.9
		Total	2.19
	LMW-GS QMENSHIP-type ⁸ (Glu-B3h)
314	LMW-GS Bu-2/12/13 [GenBank:ABC84366]	2	1.35	0.163	44	38153	8.2
317	LMW-GS Bu-2/12/13 [GenBank:ABC84366]	3	0.35	0.017	45	38153	8.2
318	LMW-GS Bu-2/12/13 [GenBank:ABC84366]	3	0.34	0.046	33	38153	8.2
322	LMW-GS Bu-2/12/13 [GenBank:ABC84366]	2	0.37	0.008	28	38153	8.2
119	LMW-GS Bu-3/[GenBank:BAD12055]	1	2.40	0.354	79	42589	8.5
119a	LMW-GS Bu-3/[GenBank:BAD12055]	2	0.69	0.256	62	42589	8.5
120	LMW-GS Bu-3/[GenBank:BAD12055]	1	0.78	0.112	61	42589	8.5
131	LMW-GS Bu-3/[GenBank:BAD12055]	1	0.72	0.047	48	42589	8.5
132	LMW-GS Bu-3/[GenBank:BAD12055]	1	0.10	0.008	39	42589	8.5
161	LMW-GS Bu-3/[GenBank:BAD12055]	3	<0.01	0.000	23	42589	8.5
237	LMW-GS Bu-3/[GenBank:BAD12055]	1	0.03	0.003	16	42589	8.5
310	LMW-GS Bu-3/[GenBank:BAD12055]	3	1.13	0.054	50	42589	8.5
316	LMW-GS Bu-3/[GenBank:BAD12055]	3	0.21	0.033	36	42589	8.5
		Total	8.47
	LMW-GS QMET-type ⁸ (Glu-D3a)
167	LMW-GS Bu-1	4	2.10	0.037	89	33008	8.7
170	LMW-GS Bu-1	3	0.33	0.021	66	33008	8.7
173	LMW-GS Bu-6	3	0.47	0.007	78	31901	8.9
144	LMW-GS Bu-7	1	1.52	0.040	50	37700	8.2
145	LMW-GS Bu-7	2	0.78	0.021	55	37700	8.2
472	LMW-GS Bu-7	3	0.17	0.071	48	37700	8.2
343	LMW-GS Bu-8	3	0.29	0.057	61	32175	7.7
203	LMW-GS Bu-11/[GenBank:AAT3786]	2	<0.01	0.000	11	39788	8.5
315	LMW-GS Bu-11/[GenBank:AAT3786]	2	0.27	0.027	52	39788	8.5
153	LMW-GS Bu-18/TC250064	1	0.26	0.030	49	37126	8.5
155	LMW-GS Bu-18/TC250064	2	0.47	0.012	50	37126	8.5
319	LMW-GS TC11_277270	2	0.67	0.038	55	39762	8.5
		Total	7.33

Loci are indicated within parentheses.
¹Protein that matched the greatest number of peptides detected in spot by MS/MS. Accession numbers from TaGI Release 10.0 are denoted with the prefix TC and TaGI release 11.0 with the prefix TC11_. Sequences of proteins from Butte 86 are found in Additional file 2. For partial Butte 86 contigs, the accession number for the most similar complete sequence is also indicated.
^2-7Footnotes as in Table 3.
⁸N-terminal sequences predicted by Target P.

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