Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Table 5 Identification and quantification of gamma-gliadins by 2DE-MS/MS.

Spot #	Predominant protein¹	Total proteins in spot²	Spot Volume³	Std Dev⁴	MS/MS Coverage^5,8	Predicted MW^6,8	pI^7,8
325	Gamma-gliadin Bu-1 or -8	3	0.89	0.044	inc	inc	inc
326	Gamma-gliadin Bu-1	2	0.13	0.014	26	35521	7.7
160	Gamma-gliadin Bu-2	1	2.17	0.002	39	35188	8.2
166	Gamma-gliadin Bu-4⁹	3	0.41	0.024	42	32606	8.2
169	Gamma-gliadin Bu-4⁹	3	1.97	0.107	56	32606	8.2
337	Gamma-gliadin Bu-4⁹	5	0.28	0.104	63	32606	8.2
134	Gamma-gliadin Bu-5	2	0.12	0.022	37	38943	7.8
320	Gamma-gliadin Bu-5	1	2.27	0.085	50	38943	7.8
323	Gamma-gliadin Bu-5	2	0.69	0.045	56	38943	7.8
324	Gamma-gliadin Bu-5	1	0.22	0.012	55	38943	7.8
335	Gamma-gliadin Bu-6	2	0.24	0.054	47	30616	8.5
346	Gamma-gliadin Bu-6	1	1.67	0.168	49	30616	8.5
347	Gamma-gliadin Bu-6	2	<0.01	0.000	32	30616	8.5
339	Gamma-gliadin Bu-7	2	0.36	0.039	52	31010	8.2
163	Gamma-gliadin Bu-11/[GenBank:AAD30556]	1	0.38	0.037	14	32018	6.5
527	Gamma-gliadin Bu-11/[GenBank:AAD30556]	3	0.37	0.018	32	32018	6.5

¹Protein that matched the greatest number of peptides detected in spot by MS/MS. For partial Butte 86 contigs, the accession number for the most similar complete sequence is also indicated.
^2-7Footnotes as in Table 3.
⁸inc indicates that sequence was incomplete.
⁹Contains nine Cys residues.

ISSN: 1477-5956