Skip to main content

Table 5 Identification and quantification of gamma-gliadins by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot # Predominant protein1 Total
proteins
in spot2
Spot
Volume3
Std
Dev4
MS/MS
Coverage5,8
Predicted
MW6,8
pI7,8
325 Gamma-gliadin Bu-1 or -8 3 0.89 0.044 inc inc inc
326 Gamma-gliadin Bu-1 2 0.13 0.014 26 35521 7.7
160 Gamma-gliadin Bu-2 1 2.17 0.002 39 35188 8.2
166 Gamma-gliadin Bu-49 3 0.41 0.024 42 32606 8.2
169 Gamma-gliadin Bu-49 3 1.97 0.107 56 32606 8.2
337 Gamma-gliadin Bu-49 5 0.28 0.104 63 32606 8.2
134 Gamma-gliadin Bu-5 2 0.12 0.022 37 38943 7.8
320 Gamma-gliadin Bu-5 1 2.27 0.085 50 38943 7.8
323 Gamma-gliadin Bu-5 2 0.69 0.045 56 38943 7.8
324 Gamma-gliadin Bu-5 1 0.22 0.012 55 38943 7.8
335 Gamma-gliadin Bu-6 2 0.24 0.054 47 30616 8.5
346 Gamma-gliadin Bu-6 1 1.67 0.168 49 30616 8.5
347 Gamma-gliadin Bu-6 2 <0.01 0.000 32 30616 8.5
339 Gamma-gliadin Bu-7 2 0.36 0.039 52 31010 8.2
163 Gamma-gliadin Bu-11/[GenBank:AAD30556] 1 0.38 0.037 14 32018 6.5
527 Gamma-gliadin Bu-11/[GenBank:AAD30556] 3 0.37 0.018 32 32018 6.5
  1. 1Protein that matched the greatest number of peptides detected in spot by MS/MS. For partial Butte 86 contigs, the accession number for the most similar complete sequence is also indicated.
  2. 2-7Footnotes as in Table 3.
  3. 8inc indicates that sequence was incomplete.
  4. 9Contains nine Cys residues.