Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Table 6 Identification and quantification of omega-gliadins by 2DE-MS/MS.

Spot #	Predominant protein¹	Total proteins in spot²	Spot Volume³	Std Dev⁴	MS/MS Coverage^5,8	Predicted MW^6,8	pI^7,8
	Omega-gliadins ω-5, 1B type (Gli-B3h)
60	Omega-gliadin ω-5 type TC11_288652	1	1.39	0.126	14	50904	6.0
63	Omega-gliadin ω-5 type TC11_288652	1	0.19	0.025	13	50904	6.0
69	Omega-gliadin ω-5 type⁹	1	0.18	0.006	inc	inc	inc
71	Omega-gliadin ω-5 type TC11_288652	1	1.27	0.171	26	50904	6.0
73	Omega-gliadin ω-5 type⁹	1	0.28	0.028	inc	inc	inc
74	Omega-gliadin ω-5 type TC11-288652	1	1.77	0.235	30	50904	6.0
		Total	5.08
	Omega-gliadins ω-1/ω-2, 1D type (Gli-D3a)
476	Omega-gliadin Gli-D3 type¹⁰	1	2.07	0.136	inc	inc	inc
477	Omega-gliadin Bu-D1/[GenBank:AAT74547]	3	0.99	0.087	23	43525	6.0
		Total	3.06
	Omega-gliadins 1D Cys type
107	Omega-gliadin TC262770	1	0.12	0.015	8	41811	5.0
113	Omega gliadin TC262770	2	0.50	0.012	46	41811	5.0
115	Omega gliadin TC262770	2	0.47	0.065	34	41811	5.0
116	Omega gliadin TC262770	1	0.16	0.024	8	41811	5.0
		Total	1.25
	Omega-gliadins ω-1/ω-2, 1A type (Gli-A3f)
130	Omega-secalin-like [GenBank:ACN96903]	1	0.08	0.055	27	39492	7.0
135	Omega-gliadin Bu-D5	1	0.56	0.055	inc	inc	inc
391	Omega-gliadin Bu-D5	2	0.43	0.049	inc	inc	inc
		Total	1.07

Loci are indicated within parentheses.
^1-7Footnotes as in Table 4.
⁸inc indicates sequence was incomplete.
⁹ No peptides were obtained; identified by other means.
¹⁰ Beta-amylase was the predominant protein detected in spot 476 but other data indicate that this spot was predominantly omega-gliadin.

ISSN: 1477-5956