Skip to main content

Table 6 Identification and quantification of omega-gliadins by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot # Predominant protein1 Total
proteins
in spot2
Spot
Volume3
Std
Dev4
MS/MS
Coverage5,8
Predicted
MW6,8
pI7,8
  Omega-gliadins ω-5, 1B type (Gli-B3h)       
60 Omega-gliadin ω-5 type TC11_288652 1 1.39 0.126 14 50904 6.0
63 Omega-gliadin ω-5 type TC11_288652 1 0.19 0.025 13 50904 6.0
69 Omega-gliadin ω-5 type9 1 0.18 0.006 inc inc inc
71 Omega-gliadin ω-5 type TC11_288652 1 1.27 0.171 26 50904 6.0
73 Omega-gliadin ω-5 type9 1 0.28 0.028 inc inc inc
74 Omega-gliadin ω-5 type TC11-288652 1 1.77 0.235 30 50904 6.0
   Total 5.08     
  Omega-gliadins ω-1/ω-2, 1D type (Gli-D3a)       
476 Omega-gliadin Gli-D3 type10 1 2.07 0.136 inc inc inc
477 Omega-gliadin Bu-D1/[GenBank:AAT74547] 3 0.99 0.087 23 43525 6.0
   Total 3.06     
  Omega-gliadins 1D Cys type       
107 Omega-gliadin TC262770 1 0.12 0.015 8 41811 5.0
113 Omega gliadin TC262770 2 0.50 0.012 46 41811 5.0
115 Omega gliadin TC262770 2 0.47 0.065 34 41811 5.0
116 Omega gliadin TC262770 1 0.16 0.024 8 41811 5.0
   Total 1.25     
  Omega-gliadins ω-1/ω-2, 1A type (Gli-A3f)       
130 Omega-secalin-like [GenBank:ACN96903] 1 0.08 0.055 27 39492 7.0
135 Omega-gliadin Bu-D5 1 0.56 0.055 inc inc inc
391 Omega-gliadin Bu-D5 2 0.43 0.049 inc inc inc
   Total 1.07     
  1. Loci are indicated within parentheses.
  2. 1-7Footnotes as in Table 4.
  3. 8inc indicates sequence was incomplete.
  4. 9 No peptides were obtained; identified by other means.
  5. 10 Beta-amylase was the predominant protein detected in spot 476 but other data indicate that this spot was predominantly omega-gliadin.