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Table 7 Identification and quantification of alpha-gliadins by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot #

Predominant protein1

Total

proteins

in spot2

Spot

Vol3

Std

Dev4

MS/MS

Coverage5,8

Predicted

MW6,8

pI7,8

Celiac

Peptides9

 

Alpha-gliadins (Gli-A2)

       

183

Alpha-gliadin Bu-5

3

1.23

0.076

76

30506

7.1

6

344

Alpha-gliadin Bu-5

1

0.16

0.028

57

30506

7.1

6

331

Alpha-gliadin Bu-14

2

0.28

0.041

15

29995

6.2

6

190

Alpha-gliadin Bu-14

2

0.48

0.035

78

29995

6.2

6

206

Alpha-gliadin Bu-14

3

0.15

0.077

29

29995

6.2

6

546

Alpha-gliadin Bu-14

3

0.18

0.029

47

29995

6.2

6

  

Total

2.48

     
 

Alpha-gliadins (Gli-B2)

       

327

Alpha-gliadin Bu-11

5

1.59

0.107

30

34599

6.5

4

525

Alpha-gliadin Bu-12

4

0.56

0.097

65

31541

7.0

-

328

Alpha-gliadin Bu-12

4

0.27

0.058

25

31541

7.0

-

329

Alpha-gliadin Bu-12

4

1.59

0.172

27

31541

7.0

-

387

Alpha-gliadin Bu-12

4

0.17

0.017

49

31541

7.0

-

524

Alpha-gliadin Bu-12

4

0.47

0.032

80

31541

7.0

-

341

Alpha-gliadin Bu-23

3

3.29

0.154

59

33871

7.1

-

  

Total

7.94

     
 

Alpha-gliadins (Gli-D2)

       

342

Alpha-gliadin Bu-1

4

0.85

0.059

60

33412

7.8

1,2,3,4,5

330

Alpha-gliadin Bu-210

6

2.66

0.148

52

30807

7.7

1,2,4

338

Alpha-gliadin Bu-210

2

0.56

0.034

60

30808

7.7

1,2,4

468

Alpha-gliadin Bu-3

4

1.66

0.119

71

33156

7.0

1,2,3,4,5

467

Alpha-gliadin Bu-4

6

2.01

0.183

78

31462

6.6

1,2,3,4

550

Alpha-gliadin Bu-10

4

1.31

0.127

75

31551

6.8

1,2,3

  

Total

9.05

     
 

Alpha-gliadins (unknown locus)

       

124

Alpha-gliadin Bu [GenBank:BQ807130]

1

0.29

0.024

inc

inc

inc

inc

177

Alpha-gliadin Bu [GenBank:BQ806209]

5

0.32

0.118

inc

inc

inc

inc

420

Alpha-gliadin Bu-27

3

0.34

0.055

55

34268

8.3

-

  

Total

0.96

     
 

Gliadin mixed spots 11

       

334

3 gamma-gliadins, 1 alpha-gliadin

4

1.08

0.105

nd

nd

nd

nd

389

1 alpha-gliadin, 1 other protein

2

0.14

0.009

nd

nd

nd

nd

172

1 gamma-gliadin, 2 alpha-gliadins

3

1.21

0.100

nd

nd

nd

nd

530

1 globulin, 1 alpha-gliadin

2

0.08

0.019

nd

nd

nd

nd

195

1 farinin, 1 alpha-gliadin, 1 enzyme

3

0.05

0.018

nd

nd

nd

nd

  

Total

2.56

     
  1. Loci are indicated within parentheses.
  2. 1-7 Footnotes as in Table 3.
  3. 8inc indicates sequence was incomplete. nd indicates not determined because the spot contained a mixture of proteins.
  4. 9 The numbers indicate the presence of one or more of the following peptide sequences associated with celiac disease: 1, glia-α-9 epitope, core sequence PFPQPQLPY; 2, glia-α-20 epitope, core sequence PQPQLPYPQ; 3, glia-α-20 epitope, core sequence RPQQPYPQ; 4, glia-α epitope, core sequence QGSFQPSQQ; 5, 33-mer toxic fragment, LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF; 6, p31-43, LGQQQPFPPQQPY. - indicates that the alpha-gliadin does not contain any of these sequences.
  5. 10Contains seven Cys residues.
  6. 11No single protein had a predominant number of peptides. Details are in Additional file 1.
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