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Table 7 Identification and quantification of alpha-gliadins by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot # Predominant protein1 Total
proteins
in spot2
Spot
Vol3
Std
Dev4
MS/MS
Coverage5,8
Predicted
MW6,8
pI7,8 Celiac
Peptides9
  Alpha-gliadins (Gli-A2)        
183 Alpha-gliadin Bu-5 3 1.23 0.076 76 30506 7.1 6
344 Alpha-gliadin Bu-5 1 0.16 0.028 57 30506 7.1 6
331 Alpha-gliadin Bu-14 2 0.28 0.041 15 29995 6.2 6
190 Alpha-gliadin Bu-14 2 0.48 0.035 78 29995 6.2 6
206 Alpha-gliadin Bu-14 3 0.15 0.077 29 29995 6.2 6
546 Alpha-gliadin Bu-14 3 0.18 0.029 47 29995 6.2 6
   Total 2.48      
  Alpha-gliadins (Gli-B2)        
327 Alpha-gliadin Bu-11 5 1.59 0.107 30 34599 6.5 4
525 Alpha-gliadin Bu-12 4 0.56 0.097 65 31541 7.0 -
328 Alpha-gliadin Bu-12 4 0.27 0.058 25 31541 7.0 -
329 Alpha-gliadin Bu-12 4 1.59 0.172 27 31541 7.0 -
387 Alpha-gliadin Bu-12 4 0.17 0.017 49 31541 7.0 -
524 Alpha-gliadin Bu-12 4 0.47 0.032 80 31541 7.0 -
341 Alpha-gliadin Bu-23 3 3.29 0.154 59 33871 7.1 -
   Total 7.94      
  Alpha-gliadins (Gli-D2)        
342 Alpha-gliadin Bu-1 4 0.85 0.059 60 33412 7.8 1,2,3,4,5
330 Alpha-gliadin Bu-210 6 2.66 0.148 52 30807 7.7 1,2,4
338 Alpha-gliadin Bu-210 2 0.56 0.034 60 30808 7.7 1,2,4
468 Alpha-gliadin Bu-3 4 1.66 0.119 71 33156 7.0 1,2,3,4,5
467 Alpha-gliadin Bu-4 6 2.01 0.183 78 31462 6.6 1,2,3,4
550 Alpha-gliadin Bu-10 4 1.31 0.127 75 31551 6.8 1,2,3
   Total 9.05      
  Alpha-gliadins (unknown locus)        
124 Alpha-gliadin Bu [GenBank:BQ807130] 1 0.29 0.024 inc inc inc inc
177 Alpha-gliadin Bu [GenBank:BQ806209] 5 0.32 0.118 inc inc inc inc
420 Alpha-gliadin Bu-27 3 0.34 0.055 55 34268 8.3 -
   Total 0.96      
  Gliadin mixed spots 11        
334 3 gamma-gliadins, 1 alpha-gliadin 4 1.08 0.105 nd nd nd nd
389 1 alpha-gliadin, 1 other protein 2 0.14 0.009 nd nd nd nd
172 1 gamma-gliadin, 2 alpha-gliadins 3 1.21 0.100 nd nd nd nd
530 1 globulin, 1 alpha-gliadin 2 0.08 0.019 nd nd nd nd
195 1 farinin, 1 alpha-gliadin, 1 enzyme 3 0.05 0.018 nd nd nd nd
   Total 2.56      
  1. Loci are indicated within parentheses.
  2. 1-7 Footnotes as in Table 3.
  3. 8inc indicates sequence was incomplete. nd indicates not determined because the spot contained a mixture of proteins.
  4. 9 The numbers indicate the presence of one or more of the following peptide sequences associated with celiac disease: 1, glia-α-9 epitope, core sequence PFPQPQLPY; 2, glia-α-20 epitope, core sequence PQPQLPYPQ; 3, glia-α-20 epitope, core sequence RPQQPYPQ; 4, glia-α epitope, core sequence QGSFQPSQQ; 5, 33-mer toxic fragment, LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF; 6, p31-43, LGQQQPFPPQQPY. - indicates that the alpha-gliadin does not contain any of these sequences.
  5. 10Contains seven Cys residues.
  6. 11No single protein had a predominant number of peptides. Details are in Additional file 1.