Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Dupont, Frances M; Vensel, William H; Tanaka, Charlene K; Hurkman, William J; Altenbach, Susan B

doi:10.1186/1477-5956-9-10

Proteome Science

Table 9 Identification and quantification of alpha-amylase and protease inhibitors by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot #	Predominant protein¹	Total proteins in spot²	Spot Volume³	Std Dev⁴	MS/MS Coverage^5,8	Predicted MW^6,8	pI^7,8
	Alpha-amylase and protease inhibitors
290	CMx1/CMx3 TC11_308146	2	0.03	0.004	57	14027	8.1
281	CMx1/CMx3 TC11_309398	3	0.23	0.029	60	13891	8.0
244	WASI [SwissProt: P16347]	1	0.17	0.027	73	19633	6.8
277	WCI [GenBank:CAD19440]	1	0.12	0.023	60	12943	7.4
283	WDAI TC11_338524	3	0.22	0.006	68	13239	5.7
286	WDAI [GenBank:AAV91972]	5	0.12	0.020	77	13191	5.2
312	WDAI [SwissProt:P01085]	3	0.67	0.125	74	13337	6.7
289	WMAI [PRF:223520]	1	0.37	0.032	72	13342	6.2
528	WMAI [PRF:223520]	1	0.13	0.022	74	13342	6.2
313	WTAI-CM1 TC11_340510	4	0.21	0.014	53	13096	6.7
285	WTAI-CM2 [SwissProt:P16851]	3	0.05	0.020	42	13034	6.2
280	WTAI-CM2 [SwissProt:P16851]	3	0.43	0.025	57	13034	6.2
264	WTAI-CM3 [SwissProt:P17314]	2	0.40	0.016	90	15832	6.7
265	WTAI-CM3 [SwissProt:P17314]	2	0.09	0.032	65	15832	6.7
266	WTAI-CM16 [SwissProt:P16159]	1	0.04	0.004	53	13437	5.0
284	WTAI-CM16 [SwissProt:P16159]	1	0.48	0.021	76	13437	5.0
274	WTAI-CM17 [GenBank:CAA42453]	1	0.02	0.004	20	13502	4.9
282	WTAI-CM17 [GenBank:CAA42453]	2	0.20	0.008	56	13502	4.9
278⁹	WCI [GenBank:CAD19440]/wheatwin-Bu-2/trypsin inhibitor factor TC11_315743	3	0.14	0.025	nd	nd	nd
	Serpins
146	Serpin Bu-1 Type 1b, like [GenBank:ACN59483]	1	0.07	0.004	53	37667	5.4
147	Serpin Bu-1 Type 1b, like [GenBank:ACN59483]	1	0.09	0.006	64	37667	5.4
148	Serpin Bu-1 Type 1b, like [GenBank:ACN59483]	1	0.44	0.015	57	37667	5.4
158	Serpin Bu-1 or Bu-4	1	0.02	0.011	inc	inc	inc
162	Serpin Bu-1 or Bu-4	1	0.02	0.005	inc	inc	inc
149	Serpin Bu-2 Serpin Z1c, like [SwissProt:Q9ST58]	2	0.17	0.012	70	42882	5.6
397	Serpin Bu-2 Serpin Z1c, like [SwissProt:Q9ST58]	1	0.02	0.004	18	42882	5.6
398	Serpin Bu-3, Z1a type [Swiss-Prot: P93693]	1	0.04	0.006	40	43118	5.6
399	Serpin Bu-3, Z1a type [Swiss-Prot: P93693]	1	0.04	0.007	24	43118	5.6
159	Serpin Bu-4 or Bu-5	3	0.24	0.007	inc	inc	inc
151	Serpin Bu-5, like [GenBank: CAA72274]	1	0.19	0.016	62	42981	5.2
154	Serpin Bu-5, like [GenBank: CAA72274]	1	0.08	0.011	23	42981	5.2
150	Serpin Bu-7, like [GenBank:ACN59484]	1	0.10	0.010	62	43431	5.1
152	Serpin Bu-7, like [GenBank:ACN59484]	1	0.06	0.016	39	43431	5.1
	Other Inhibitors
201	Tritin TC235992	4	0.15	0.017	64	29653	9.8
205	Xylanase inhibitor XIP-1 [PDB:1OM0]	1	0.11	0.042	54	30285	8.3

^1-7 Footnotes as in Table 4.
⁸ inc indicates the sequence was incomplete. nd indicates not determined because no single protein had a predominant number of peptides.
⁹ Spot contained a similar number of peptides from three different inhibitors. Details are in Additional file 1.

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ISSN: 1477-5956

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