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Table 9 Identification and quantification of alpha-amylase and protease inhibitors by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot # Predominant protein1 Total
proteins
in spot2
Spot
Volume3
Std
Dev4
MS/MS
Coverage5,8
Predicted
MW6,8
pI7,8
  Alpha-amylase and protease inhibitors       
290 CMx1/CMx3 TC11_308146 2 0.03 0.004 57 14027 8.1
281 CMx1/CMx3 TC11_309398 3 0.23 0.029 60 13891 8.0
244 WASI [SwissProt: P16347] 1 0.17 0.027 73 19633 6.8
277 WCI [GenBank:CAD19440] 1 0.12 0.023 60 12943 7.4
283 WDAI TC11_338524 3 0.22 0.006 68 13239 5.7
286 WDAI [GenBank:AAV91972] 5 0.12 0.020 77 13191 5.2
312 WDAI [SwissProt:P01085] 3 0.67 0.125 74 13337 6.7
289 WMAI [PRF:223520] 1 0.37 0.032 72 13342 6.2
528 WMAI [PRF:223520] 1 0.13 0.022 74 13342 6.2
313 WTAI-CM1 TC11_340510 4 0.21 0.014 53 13096 6.7
285 WTAI-CM2 [SwissProt:P16851] 3 0.05 0.020 42 13034 6.2
280 WTAI-CM2 [SwissProt:P16851] 3 0.43 0.025 57 13034 6.2
264 WTAI-CM3 [SwissProt:P17314] 2 0.40 0.016 90 15832 6.7
265 WTAI-CM3 [SwissProt:P17314] 2 0.09 0.032 65 15832 6.7
266 WTAI-CM16 [SwissProt:P16159] 1 0.04 0.004 53 13437 5.0
284 WTAI-CM16 [SwissProt:P16159] 1 0.48 0.021 76 13437 5.0
274 WTAI-CM17 [GenBank:CAA42453] 1 0.02 0.004 20 13502 4.9
282 WTAI-CM17 [GenBank:CAA42453] 2 0.20 0.008 56 13502 4.9
2789 WCI [GenBank:CAD19440]/wheatwin-Bu-2/trypsin inhibitor factor TC11_315743 3 0.14 0.025 nd nd nd
  Serpins       
146 Serpin Bu-1 Type 1b, like [GenBank:ACN59483] 1 0.07 0.004 53 37667 5.4
147 Serpin Bu-1 Type 1b, like [GenBank:ACN59483] 1 0.09 0.006 64 37667 5.4
148 Serpin Bu-1 Type 1b, like [GenBank:ACN59483] 1 0.44 0.015 57 37667 5.4
158 Serpin Bu-1 or Bu-4 1 0.02 0.011 inc inc inc
162 Serpin Bu-1 or Bu-4 1 0.02 0.005 inc inc inc
149 Serpin Bu-2 Serpin Z1c, like [SwissProt:Q9ST58] 2 0.17 0.012 70 42882 5.6
397 Serpin Bu-2 Serpin Z1c, like [SwissProt:Q9ST58] 1 0.02 0.004 18 42882 5.6
398 Serpin Bu-3, Z1a type [Swiss-Prot: P93693] 1 0.04 0.006 40 43118 5.6
399 Serpin Bu-3, Z1a type [Swiss-Prot: P93693] 1 0.04 0.007 24 43118 5.6
159 Serpin Bu-4 or Bu-5 3 0.24 0.007 inc inc inc
151 Serpin Bu-5, like [GenBank: CAA72274] 1 0.19 0.016 62 42981 5.2
154 Serpin Bu-5, like [GenBank: CAA72274] 1 0.08 0.011 23 42981 5.2
150 Serpin Bu-7, like [GenBank:ACN59484] 1 0.10 0.010 62 43431 5.1
152 Serpin Bu-7, like [GenBank:ACN59484] 1 0.06 0.016 39 43431 5.1
  Other Inhibitors       
201 Tritin TC235992 4 0.15 0.017 64 29653 9.8
205 Xylanase inhibitor XIP-1 [PDB:1OM0] 1 0.11 0.042 54 30285 8.3
  1. 1-7 Footnotes as in Table 4.
  2. 8 inc indicates the sequence was incomplete. nd indicates not determined because no single protein had a predominant number of peptides.
  3. 9 Spot contained a similar number of peptides from three different inhibitors. Details are in Additional file 1.