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Table 1 List of differentially expressed protein spots in tracheal tissues identified by MALDI-TOF-TOF MS and MS/MS analysis

From: Proteomic analysis of chicken embryonic trachea and kidney tissues after infection in ovo by avian infectious bronchitis coronavirus

Spota

Accession Numberb

Protein Description

Mr(KDa)/ pI

Score

Coverage (%)d

Normalized spot volume (vol%)e

p

Ratio (infected/mock-infected)

Protein functions

Other viruses found inf

      

Mock-infected

IBV-infected

    

Cytoskeletal and calcium ion-binding proteins

1

gi|55584150

Myosin light chain 3, skeletal muscle isoform [Gallus gallus]

16.7/4.52

81c

38

0.1007 ± 0.0245

0.0452 ± 0.0118

0.024

0.45

Motor activity. Calcium ion binding.

IAV, RSV, CVB3

2

gi|212347

Myosin light chain 1, skeletal muscle isoform [Gallus gallus]

19.5/4.96

192

53

0.6040 ± 0.0519

0.3576 ± 0.0656

0.007

0.59

Motor activity. Calcium ion binding.

 

16

gi|50403707

myosin light chain type 2 (LC2f) [Gallus gallus]

18.9/4.77

220

33

0.4716 ± 0.0545

0.2784 ± 0.0257

0.005

0.59

Motor activity. Calcium ion binding.

VHSV

18

gi|46195459

annexin A1 [Gallus gallus]

38.5/7.05

142c

39

0.1277 ± 0.0106

0.0596 ± 0.0118

0.002

0.47

Calcium/phospholipid-binding protein. Promotes membrane fusion. Involved in exocytosis. Regulates phospholipase A2 activity. Inflammation response

CSFV, PRRSV, HBV, RSV, VHSV, WNV

Carbohydrate and lipid metabolic proteins

3

gi|20178282

Extracellular fatty acid-binding protein [Gallus gallus]

20.1/5.56

361

46

0.0495 ± 0.0094

0.1058 ± 0.0221

0.015

2.14

Fatty acid binding and transporting. Inflammatory response.

 

17

gi|45382875

Creatine kinase M chain [Gallus gallus]

43.3/6.5

287

50

0.5632 ± 0.0122

0.2546 ± 0.0488

< 0.001

0.45

Nucleotide binding. Catalytic activity. Creatine kinase activity

HBV, VHSV

Stress response protein

15

gi|45384222

Heat shock 27 kDa protein [Gallus gallus]

21.7/5.77

166

61

0.2906 ± 0.0637

0.1531 ± 0.0432

0.036

0.53

Response to stress. Anti-apoptosis

CSFV, PRRSV, PCV2, ASFV, AIV, MDV, IBDV, REOV, 1AV, CVB3

Protein and nucleotide processing

5

gi|122692295

ubiquitin carboxyl-terminal esterase L1 [Gallus gallus]

25.1/5.74

142

31

0.0563 ± 0.0019

0.0980 ± 0.0071

0.001

1.74

Ubiquitin binding, Protein deubiquitination

DHBV, IBDV

6

gi|45382983

replication factor C (activator 1) 2, 40 kDa [Gallus gallus]

40.1/5.68

87c

45

0.0542 ± 0.0128

0.1931 ± 0.0297

0.002

3.56

Nucleotide binding. ATP binding

 

10

gi|52138673

chaperonin containing TCP1, subunit 8 (theta) [Gallus gallus]

59.5/5.35

300

37

0.0403 ± 0.0031

0.0760 ± 0.0047

0.001

1.89

Protein binding. Nucleotide binding

PRRSV, EV71, HPV8, IAV

11

gi|52138673

chaperonin containing TCP1, subunit 8 (theta) [Gallus gallus]

59.5/5.35

356

47

0.0559 ± 0.0021

0.1228 ± 0.0375

0.037

2.20

Protein binding. Nucleotide binding

PRRSV, EV71, HPV8, IAV

4

gi|150247116

Putative uncharacterized protein TRIM27.2 (Tripartite motif-containing) [Gallus gallus]

27.2/5.25

289c

46

0.0792 ± 0.0116

0.2098 ± 0.0196

0.001

2.65

Protein binding. Metal ion binding

 

9

gi|45383758

cholinergic receptor, nicotinic, alpha 7 precursor [Gallus gallus]

56.9/5.47

159c

29

0.0323 ± 0.0055

0.0723 ± 0.0182

0.022

2.24

Acetylcholine receptor activity. Activation of MAPK activity. Cellular calcium ion homeostasis

 

7

gi|71896049

cholinergic receptor, nicotinic, gamma polypeptide precursor [Gallus gallus]

59.6/5.53

142c

26

0.0701 ± 0.0132

0.1207 ± 0.0039

0.003

1.72

Nicotinic acetylcholine-activated cation-selective channel activity. Ion channel activity

 

8-1

gi|45382569

ARP2 actin-related protein 2 homolog [Gallus gallus]

45.0/6.3

157c

51

0.0747 ± 0.0027

0.1336 ± 0.0187

0.006

1.79

ATP binding. Actin binding. Protein binding

 

13

gi|71895337

ovoinhibitor precursor [Gallus gallus]

54.4/6.16

128c

39

0.0334 ± 0.0139

0.1046 ± 0.0364

0.034

3.13

Serine-type endopeptidase inhibitor activity. Peptidase inhibitor activity.

 

14

gi|71895337

ovoinhibitor precursor [Gallus gallus]

54.4/6.16

291

40

0.0545 ± 0.0228

0.1074 ± 0.0119

0.029

1.97

Serine-type endopeptidase inhibitor activity. Peptidase inhibitor activity.

 

Signal transduction

12

gi|124249432

Rho GDP dissociation inhibitor (GDI) alpha [Gallus gallus]

23.3/5.22

264

60

0.1091 ± 0.0127

0.0619 ± 0.0131

0.011

0.57

Rho GDP-dissociation inhibitor activity. Signal transduction

CSFV, RSV, WSSV, YHV, IBDV

Metal ion binding

8-2

gi|17942831

Chain A, Ovotransferrin, C-Terminal Lobe, Apo Form

39.4/6.31

93c

49

0.0747 ± 0.0027

0.1336 ± 0.0187

0.006

1.79

Metal ion binding. Iron ion transport

 
  1. a) Spot ID: is the unique number which refers to the labels in Figure 2 and Figure 3
  2. b) Accession Number: gi number in NCBI.
  3. c) Score: Protein score based only on MS spectra by MALDI-TOF, other spots based on combined MS and MS/MS spectra from MALDI-TOF-TOF identification, a protein score greater than 83 is significant in this study (p < 0.05).
  4. d) Coverage (%): Percentage of identified protein sequences covered by matched peptides.
  5. e) Values are presented as mean ± SD. n = 3; vol % was defined as the ratio of the intensity volume of each spot to that of all spots calculated by the software. The SD represents standard deviation of the vol % in three biological replicates.
  6. f) IAV, Influenza A virus; RSV, Human respiratory syncytial virus; CVB3, coxsackievirus B3; VHSV, Viral haemorrhagic septicemia virus; CSFV, classical swine fever virus; PRRSV, Porcine reproductive and respiratory syndrome virus; PCV2, Porcine circovirus type 2; ASFV, African swine fever virus; HBV, Hepatitis B virus; AIV, Avian influenza virus; EV71, Enterovirus 71; MDV, Marek's disease virus; WSSV, White spot syndrome virus; DHBV, Duck heptatitis B virus; YHV, yellow head virus; HPV8, Human papillomavirus type 8; REOV, Reovirus; IBDV, Infectious bursal disease virus.