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Table 4 Time-dependent downregulated protein spots in response to Pb

From: Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure

   

Treatment/control ratio

  

KOG Class

Protein ID

Putative Function

1h

2h

4h

8h

Subcellular Location*

Multiple spots

Amino acid transport and metabolism

137931

Glycine/serine hydroxymethyltransferase

0,56

0,27

0,19

0,42

C

3

 

1542

3-isopropylmalate dehydrogenase

0,39

0,42

0,26

0,39

C

-

 

10011

3-isopropylmalate dehydratase (aconitase superfamily)

0,15

0,55

0,14

0,18

Cysk

-

 

125842

Lysine-ketoglutarate reductase/saccharopine dehydrogenase

0,43

0,17

0,08

0,33

C

-

 

131837

Oxoprolinase

0,56

0,32

0,31

0,24

C

-

 

134775

Glutamine amidotransferase/cyclase

0,53

0,67

0,59

0,26

C

-

 

138721

Glutamate/leucine/phenylalanine/valine dehydrogenases

0,84

0,35

0,23

0,34

C

2

 

139663

Methionine synthase II (cobalamin-independent)

0,25

0,36

0,05

0,17

C

5

Carbohydrate transport and metabolism

8743

Inositol monophosphatase

0,12

0,04

0,06

0,39

C

-

 

10433

Transketolase

0,92

0,89

0,89

0,18

C

-

 

133884

Glycolipid transfer protein

0,04

1,12

0,39

0,27

C

-

 

137623

Phosphoglucomutase

1,54

0,35

0,17

1,07

C

-

 

122435c

3-phosphoglycerate kinase

0,95

0,31

0,24

1,59

C

3

 

3052a

Mannose-6-phosphate isomerase, type II

0,44

0,54

0,60

0,29

Cysk

2

 

3052b

Mannose-6-phosphate isomerase, type II

0,91

0,44

0,18

0,32

Cysk

2

Coenzyme transport and metabolism

10308

S-adenosylhomocysteine hydrolase

0,67

0,64

0,30

0,56

C

-

Cytoskeleton

139298

Actin and related proteins

0,30

0,37

0,07

0,11

Cysk

4

Defense mechanism

10742

N-6 Adenine-specific DNA methylase

1,05

0,6

0,32

0,19

Mit

-

Energy production and conversion

912

Kynurenine 3-monooxygenase and related flavoprotein monooxygenases

0,78

0,41

0,19

0,20

Ext

-

 

1350

Aldehyde dehydrogenase

0,45

0,34

0,36

0,29

C

-

 

132162

NAD-dependent malate dehydrogenase

0,00

0,37

0,55

0,51

C

-

 

132918

Sulfide:quinone oxidoreductase/flavo-binding protein

0,85

0,49

0,22

0,55

Mit

-

 

134368

Vacuolar H+-ATPase V1 sector, subunit E

0,29

0,67

0,32

1,98

Nuc

-

 

135659

NADP+-dependent malic enzyme

1,13

0,72

0,18

1,18

Mit

-

 

140211

Glyoxylate/hydroxypyruvate reductase (D-isomer-specific 2-hydroxy acid dehydrogenase superfamily)

1,51

0,51

0,29

0,33

C

-

 

123932b

Fumarate reductase, flavoprotein subunit

1,21

0,85

0,60

0,10

C

2

 

131257a

NADH-ubiquinone oxidoreductase, NDUFS1/75 kDa subunit

0,91

0,88

0,42

0,21

C

3

 

131879a

Dihydrolipoamide dehydrogenase

0,62

0,52

0,26

0,80

C

2

 

133924a

Aldehyde dehydrogenase

0,65

0,49

0,26

0,61

C

2

 

133924b

Aldehyde dehydrogenase

0,31

0,48

0,28

0,55

C

2

 

563a

Pyruvate dehydrogenase E1, alpha subunit

1,33

0,28

0,27

0,28

Mit

2

General function prediction only

3442

Predicted NAD-dependent oxidoreductase

0,30

0,22

0,54

0,50

C

-

 

132767b

Serine/threonine protein kinase, active site

0,55

0,85

0,55

0,27

Mit

2

Lipid transport and metabolism

511

Enoyl-CoA hydratase

1,04

0,74

0,14

0,96

C

-

 

10355b

Mevalonate pyrophosphate decarboxylase

0,73

0,36

0,24

0,48

Mit

2

Posttranslational modification, protein turnover, chaperones

361

26S proteasome regulatory complex, ATPase RPT3

0,25

0,17

0,06

0,05

Nuc

-

 

1324

Molecular co-chaperone STI1

1,02

0,68

0,01

1,11

C

-

 

1846

Chaperonin complex component, TCP-1 beta subunit (CCT2)

0,79

0,85

0,46

0,28

C

-

 

5061

Dipeptidyl aminopeptidase

1,06

1,07

0,99

0,29

Ext

2

 

8527

Thioredoxin reductase

0,27

0,45

0,22

0,31

Mit

-

 

130274

Glutathione peroxidase

0,33

0,77

1,10

0,19

C

-

 

131571

Protein disulfide isomerase (prolyl 4-hydroxylase beta subunit)

0,91

0,08

0,74

0,06

Ext

-

 

133185

26S proteasome regulatory complex, ATPase RPT1

0,31

0,34

0,07

0,04

Nuc

-

 

133717

20S proteasome, regulatory subunit alpha type PSMA4/PRE9

0,30

0,62

0,82

0,73

Mit

-

 

134073

Chaperonin complex component, TCP-1 zeta subunit (CCT6

0,70

0,42

0,14

0,10

C

-

 

139500

Multifunctional chaperone (14-3-3 family)

0,27

0,38

0,20

0,47

Nuc

-

 

10340a

HSP70(putative ortholog to S. cerevisiae Heat shock protein homolog SSE1 (Chaperone protein MSI3)

0,66

0,54

0,06

0,64

C

2

 

10340b

HSP70(putative ortholog to S. cerevisiae Heat shock protein homolog SSE1 (Chaperone protein MSI3)

0,34

0,15

0,20

0,26

C

2

Secondary metabolites biosynthesis, transport and catabolism

133231

Predicted dehydrogenase

0,26

0,53

0,16

0,10

C

-

 

4796b

Alcohol dehydrogenase, class V

0,61

0,31

0,17

0,07

C

4

Translation, ribosomal structure and biogenesis

3216

Prolyl-tRNA synthetase

0,80

0,60

0,51

0,12

C

-

 

6570

Mitochondrial translation elongation factor Tu

0,61

0,59

0,16

0,76

Mit

-

 

10819

Elongation factor 2

1,27

0,65

0,15

0,14

C

-

  1. *C; cytoplasmic, Ext; extracellular, Mit; mitochondrial, Nuc; Nuclear, Cysk; cytoskeleton, Cyto_nucl; cytoplasmic_nuclear
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Proteome Science

ISSN: 1477-5956