Differential analysis of glioblastoma multiforme proteome by a 2D-DIGE approach

Collet, Brigitte; Guitton, Nathalie; Saïkali, Stephan; Avril, Tony; Pineau, Charles; Hamlat, Abderrahmane; Mosser, Jean; Quillien, Véronique

doi:10.1186/1477-5956-9-16

Proteome Science

Table 1 Differentially expressed proteins in GBM samples identified by MALDI mass spectrometry after DIGE analysis*

From: Differential analysis of glioblastoma multiforme proteome by a 2D-DIGE approach

Average ratio GBM/NT(a)	T-test (b)	Protein name	Species	Mt Th.( c)	PI Th. (d)	Accession No. (gi) (e)	Matched Peptides (f)	Coverage % (g)	Score(h)
7.33	0,0060	hemoglobin	Homo Sapiens	15834	6.76	61679606	11/82	80	100
6.52	0.0065	dihydropyrimidinase -related protein 2	Homo Sapiens	62711	5.95	4503377	24/73	55	225
6.49	0,0058	apolipoprotein A-1	Homo Sapiens	28061	5.27	90108666	17/69	50	153
6.47	0,035	human serum albumin	Homo Sapiens	67174	5.57	55669910	26/59	54	263
6.19	0,0078	alpha-2-globin	Homo Sapiens	15174	8.73	1335076	9/55	69	86
5.88	0.024	tubulin beta 2	Homo Sapiens	50274	4.78	4507729	22/73	43	207
5.83	0,0015	ubiquitin carboxyl-terminal hydrolase isozyme L1	Homo Sapiens	25151	5.33	21361091	10/40	59	131
5.83	0,0022	ER-60 protease	Homo Sapiens	57160	5.98	1208427	17/40	34	170
4.60	0,0085	MTHSP75 (GRP-75)	Homo Sapiens	74019	5.9	292059	26/72	49	208
4.29	0.020	dihydropyrimidinase-related protein 3	Homo Sapiens	62323	6.11	4503379	12/29	27	76
4.26	0,048	vimentin variant	Homo Sapiens	53708	5.06	62896523	25/100	59	172
		higly similar to glial fibrillary acid protein (GFAP)	Homo Sapiens	49533	5.84	34536332	26/100	63	169
4.14	0,040	ACTB protein	Homo Sapiens	40536	5.55	15277503	12/57	45	93
4.02	0.050	transthyretin variants	Homo Sapiens	13806	5.57	2098255	10/42	81	153
3.59	0,049	human manganese superoxide dismutase mutantQ143n (MnSOD)	Homo Sapiens	22290	6.86	2780818	10/59	46	95
3.57	0,034	glyceraldehyde-3 phosphate dehydrogenase	Homo Sapiens	24776	8.68	89573929	8/39	47	89
3.32	0,021	heat shock protein 27	Homo Sapiens	22427	7.83	662841	10/67	57	99
2.83	0,050	mitochondrial aldehyde dehydrogenase	Homo Sapiens	54394	5,6	6137677	17/71	43,3	134
2.76	0,037	tubulin alpha -1C	Homo Sapiens	50548	4.96	14389309	15/66	49	138
2.76	0,0084	tubulin alpha	Homo Sapiens	33321	5.86	37492	8/30	22	71
2.74	0,040	glutamate carboxypeptidase	Homo Sapiens	52700	5.7	15620780	14/40	38	143
2.57	0,024	LAP 3 protein (leucyl aminopeptidase)	Homo Sapiens	54724	6.80	37588925	15/46	38	149

* CyDye images were analyzed by BVA and spots that showed statistically significant differences (Student's t-test) in intensity between the control and the GBM groups are listed. (a) Average ratio GBM/NT: The average ratio value indicates the standardized volume ratio between the two groups: GBM and non tumorous patients. A two-fold increase is represented by 2. (b) T-test: Student's T-test p value: Only proteins with ratios showing significant differences (p£0.05) were retained. (c):Mt th: Theoritical relative mass. (d) PI th: Theoritical isoelectric point. (e) GeneBank sequence identification number(f): Number of matched peptides versus total number of peptides. (g): Percent coverage of the protein. (h) Mascot score: Protein scores greater than 81 are significant (p < 0.05).

Back to article page

ISSN: 1477-5956

Contact us

General enquiries: journalsubmissions@springernature.com