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Figure 3 | Proteome Science

Figure 3

From: Identification of low-abundance proteins via fractionation of the urine proteome with weak anion exchange chromatography

Figure 3

Western blot analysis of immunoglobulins, zinc-alpha-2-glycoprotein and retinol-binding protein 4 in urine proteome and fractions of urine proteome of healthy people. (A) CBR staining patterns of total urine protein (1), fraction Unbound (2), fraction NaCl-1 (3), fraction NaCl-2 (4) and fraction NaCl-3 (5). (B) Immunoreactivity to mouse anti-human immunoglobulin antibody. Two bands, indicating immunoglobulin heavy and light chain, appeared only in 2 (fraction Unbound) and total urine protein. (C) Immunoreactivity to mouse anti-human zinc-alpha-2-glycoprotein antibody. The band appeared only in 3 (fraction NaCl-1) and total urine protein. (D) Immunoreactivity to mouse anti-human retinol-binding protein 4 antibody. The band appeared only in 3 (fraction NaCl-1) and total urine protein.

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Proteome Science

ISSN: 1477-5956