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Figure 6 | Proteome Science

Figure 6

From: Deglycosylation and label-free quantitative LC-MALDI MS applied to efficient serum biomarker discovery of lung cancer

Figure 6

Immunoblot verification of complement C3dg serum levels in the study subjects. (A) Detection of 39 kDa C3dg fragment from 0.02 μL of crude serum using anti-C3 antibody. Lanes represent 10 subjects each of normal control, early stage lung cancer (stage-I/II) and advanced stage lung cancer (stage-IIIb/IV). (B) Dot plot of the data shown in (A), quantified as the ratio of C3dg detection level to the normalization lane M, a 40 kDa size marker that has cross-reactivity with the secondary antibody.

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