2-DE profiles of urine samples. Pooled urine samples from 24 healthy individuals (A), 24 patients with low malignant BTCC (B) and 16 patients with aggressive BTCC (C) were resolved on 2-DE gels. Protein spots were visualized using CBB. Spots with more than 10-fold difference of two matched spots from two different groups were numbered. The red arrows labeled with numbers indicate the position of the differentially expressed proteins.