Figure 2
From: Identification of Apo-A1 as a biomarker for early diagnosis of bladder transitional cell carcinoma
Western blot validation of the proteins identified by 2-DE coupled with MS. Fb, LDHB, Apo-A1, CLU and Hp expression in pooled urine samples from healthy individuals, patients with low malignant or aggressive BTCC were further detected by Western blot. Equal protein amount (25 μg) from each group was loaded onto 10% SDS-PAGE gels. Antibodies were accepted as detecting a single predominant band at the expected molecular weights.