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Figure 1 | Proteome Science

Figure 1

From: Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae

Figure 1

2-D DIGE of "detergent-wash" proteins from A. pleuropneumoniae serotypes 1, 2 and 5 (subunit vaccine) and serotype 7. For analysis of the subunit vaccine serotype 1, 2, and 5 were labelled with Cy2 (shown in blue), Cy3 (shown in green) and Cy5 (shown in red), respectively, and subsequently pooled. For visualization of differences between the serotypes used for vaccine generation, we compared couples of serotypes 1 and 2 (A), serotypes 1 and 5 (B), and serotypes 2 and 5 (C). For comparison of the subunit vaccine to serotype 7, the subunit vaccine was labelled with Cy3 (shown in green) and the "detergent wash" of serotype 7 was labelled with Cy5 (shown in red, D). Spots with intensities considerably above that in one or the other serotype were analyzed by mass spectrometry from preparative gels of the respective serotype (Additional file 4, Figure S2). The annotation of the identified protein is given in the same colour as the labelling of the respective serotype. The numbers on each spot, that has been identified, are consecutive and allow the finding of the respective spot on preparative gels (Additional file 4, Figure S2).

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