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Figure 2 | Proteome Science

Figure 2

From: Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae

Figure 2

Immunoblot analysis for the identification of cross-reactive proteins. "Detergent-wash" proteins were separated by 2-D and subsequently Western blotted. "Detergent-wash" proteins from serotype 7 were probed using immune sera of pigs immunized with the subunit vaccine (A) and the subunit vaccine was probed with convalescent sera from pigs upon experimental infection with serotype 7 (B). Immunogenic spots were assigned to spots on preparative Coomassie stained gels (Additional file 4, Figure S2) and identified by mass spectrometry. The numbers on each spot that has been identified allow the finding of the respective spot on preparative gels (Additional file 4, Figure S2).

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