Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae

Buettner, Falk FR; Konze, Sarah A; Maas, Alexander; Gerlach, Gerald F

doi:10.1186/1477-5956-9-23

Proteome Science

Table 1 List of the "abundant protein fraction" identified in the A. pleuropneumoniae "detergent wash" preparations upon 1-D PAGE.

From: Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae

Protein description ^a)	Protein ^a)	Accession # ^a)	Peptide matches ^b)				PSORTb localization ^c)	PSORTb score ^c)	Characteristics ^d)
			Subunit vaccine
			Ser 1	Ser 2	Ser 5	Ser 7
RTX toxin protein	ApxIA	Q548V0	24	(1) ^e)	29	(1) ^e)	Extracellular	10	Immunogenic [40]
RTX toxin protein	ApxIIIA	P55130	(2) ^e)	26	(1) ^e)	(1) ^e)	Extracellular	10	Immunogenic [13]
Iron regulated outer membrane protein B	FrpB	B3H0B8	18	15	21	17	OuterMembrane	10	Immunogenic [13]
Outer membrane lipoprotein A	OmlA	B3GYZ9	19	14	10	19	OuterMembrane	9.92	Immunogenic [42]
Transferrin binding protein	TbpB	B3GYQ1	14	14	19	17	OuterMembrane	9.49	Immunogenic [41]
RTX toxin protein	ApxIVA	B3H1M8	10	16	15	0	Extracellular	10	Immunogenic [30]; expressed in vivo [34]; upregulated in vivo [36]
Transferrin binding protein 1 Tbp1	TbpA	B3GYQ0	7	4	16	11	OuterMembrane	10	Immunogenic [41]; expressed in vivo [34]
Elongation factor Tu	TufB1, TufB2	B3GYJ3	16	14	16	15	Cytoplasmic	9.97	Immunogenic [13]; expressed in vivo [34, 35]
Outer membrane protein P5	OmpA1	B3H2D9	9	9	14	10	OuterMembrane	10	Immunogenic [13]; expressed in vivo [34]; in vivo survival [37]; antigenic and in vivo expressed [14]
Elongation factor G	FusA	B3H2G7	4	12	12	6	Cytoplasmic	9.97
Outer membrane protein P5 OMP P5	OmpA2	B3GZA8	10	11	10	7	OuterMembrane	10	Immunogenic [13]; expressed in vivo [34]; in vivo survival [37]; antigenic and in vivo expressed [14]
Hemoglobin binding protein A	HgbA	B3H1S8	10	6	0	9	OuterMembrane	10	Expressed in vivo [34]
Outer membrane protein P2	OmpP2	B3H172	10	9	10	8	OuterMembrane	10	in vivo survival [38]
Iron Chelated ABC transporter periplasmic binding protein	YfeA	B3H0B4	6	9	10	10	Periplasmic	10	Immunogenic [13]

Protein identifications in the individual "detergent washes" of the A. pleuropneumoniae serotypes 1, 2 and 5 used for generation of the subunit vaccine ("whole vaccine-proteome approach", highlighted in bold) and serotype 7.
^a) In order to have a consistent nomenclature for proteins identified from A. pleuropneumoniae serotypes 1, 2, 5 or 7 in the columns "protein description", "protein" and "accession number", the annotation of the respective homologue of A. pleuropneumoniae serotype 7 was adopted from the uniprot knowledgebase http://www.uniprot.org/. For the Apx toxins ApxIA and ApxIIIA, which are not present in A. pleuropneumoniae serotype 7, the common nomenclature was used.
^b) Peptide matches in the serotype specific vaccine components identified by UPLC-coupled Q-TOF MS/MS
^c) Subcellular prediction using the PSORTb v.3.0 tool at http://www.psort.org/psortb/[53]. A probability based score from 0 (low probability) to 10 (high probability) is given.
^d) The identified proteins were searched in the literature for homologues identified in A. pleuropneumoniae as immunogenic, virulence-associated or relevant in vivo.
^e) These peptides from Apx toxins that are not present in the respective preparations because they are not encoded by the respective serotype were merely identified because they are identical or highly homologous to peptides from other Apx toxins that are present in the respective preparations.

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ISSN: 1477-5956

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