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Table 2 Identified proteins from the strawberry stolon I-2 internode

From: Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons

Spot No. a

Protein

Accession Number b

Molecular Function c

Reference Organism

Theoretical kDa/pI d

Experimental kDa/pI e

Score f

SC g (%)

Matched/Unmatched queries h

 

Metabolism

        

91

Fructokinase-1

A2WXV8

ATP binding

Oryza sativa

34.9/5.1

35.8/3.4

76

20

6/59

95

Caffeic acid 3-O-methyltransferase

Q8GU25

caffeate O-methyltransferase activity

Rosa chinensis

40.1/5.6

43.7/4.4

110

35

12/30

129

Malate dehydrogenase, mitochondrial

P83373

Oxidoreductase

Fragaria ananassa

35.8/8.7

39.1/7.8

74

31

8/28

139

Glyceraldehyde-3-phosphate dehydrogenase, cytosolic

P25858

Oxidoreductase

Arabidopsis thaliana

37.0/6.6

40.1/9.3

74

29

9/27

141

Malate dehydrogenase, mitochondrial

P83373

Oxidoreductase

Fragaria ananassa

35.8/8.7

41.0/9.1

65

27

6/23

172

S-adenosylmethionine synthase 2

Q9FUZ1

ATP binding

Brassica juncea

43.3/5.3

43.9/5.9

138

44

15/36

173

S-adenosylmethionine synthase

A4PU48

ATP binding

Medicago truncatula

43.7/5.6

38.5/6.8

178

52

15/17

174

S-adenosylmethionine synthase

Q8W3Y4

ATP binding

Phaseolus lunatus

43.5/5.6

41.2/6.7

132

48

15/40

 

Energy

        

176

ATP synthase subunit beta, plastid

Q8MBG5

ATP binding

Cuscuta pentagona

53.2/5.5

53.4/6.7

61

32

10/53

 

Photosynthesis

        

17

Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 4OS

Q9T079

alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

Arabidopsis thaliana

90.3/6.1

14.2/6.3

58

25

12/66

33

Ribulose bisphosphate carboxylase large chain (Fragment) OS

P28261

magnesium ion binding

Nypa fruticans

51.5/6.2

22.3/5.1

123

23

13/61

35

Probable granule-bound starch synthase 1, chloroplastic/amyloplastic

Q9MAQ0

starch synthase activity

Arabidopsis thaliana

67.5/8.7

23.0/4.5

60

24

9/37

52

Ribulose bisphosphate carboxylase large chain (Fragment)

P28391

magnesium ion binding

Ceratopetalum gummiferum

51.3/6.2

24.0/6.9

104

22

11/63

64

Ribulose-phosphate 3-epimerase, chloroplastic

Q43157

ribulose-phosphate 3-epimerase activity

Spinacia oleracea

30.6/8.2

27.2/7.9

58

31

6/51

82

Triosephosphate isomerase, chloroplastic

Q9M4S8

triose-phosphate isomerase activity

Fragaria ananassa

33.7/7.6

31.5/5.2

154

51

16/71

90

Oxygen-evolving enhancer protein 1, chloroplastic

P26320

calcium ion binding

Solanum tuberosum

35.6/5.8

34.5/4.7

98

31

10/48

103

Coproporphyrinogen-III oxidase, chloroplastic

P35055

coproporphyrinogen oxidase activity

Glycine max

43.6/6.7

42.2/5.2

84

31

10/32

104

Glutamyl-tRNA reductase 1, chloroplastic

Q42843

NADP or NADPH binding

Hordeum vulgare

58.1/8.7

42.1/4.9

70

24

11/48

 

Transcription

        

18

Homeobox-leucine zipper protein GLABRA 2 OS

P46607

sequence-specific DNA binding

Arabidopsis thaliana

83.7/6.1

15.0/6.4

63

31

20/90

47

Protein HIRA

Q32SG6

transcription regulator activity

Zea mays

106.55/7.8

19.7/7.1

60

22

16/90

121

Two-component response regulator ARR9

O80366

two-component response regulator activity

Arabidopsis thaliana

26.2/5.2

34.6/8.1

58

35

5/43

128

DNA-directed RNA polymerase subunit beta

P12465

DNA binding

Chlorella vulgaris

179.4/9.9

36.9/8.0

60

9

11/34

149

B3 domain-containing protein Os07g0679700

Q6Z3U3

DNA binding

Oryza sativa

105.4/6.5

53.5/7.9

63

11

9/27

168

Protein HIRA

Q32SG6

transcription regulator activity

Zea mays

106.5/7.8

41.1/6.8

60

22

16/90

 

Protein synthesis

        

28

Eukaryotic translation initiation factor 5A-2 OS

Q945F4

translation initiation factor activity

Medicago sativa

17.5/5.4

17.3/4.7

74

26

5/39

37

Eukaryotic translation initiation factor 5A

Q9AXQ7

translation initiation factor activity

Dianthus caryophyllus

17.6/5.6

20.3/5.9

88

45

7/28

45

Glutathione gamma-glutamylcysteinyltransferase 2

Q9ZWB7

acyltransferase activity

Arabidopsis thaliana

52.3/6.6

16.4/6.6

57

15

8/32

66

Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic

P21357

3-deoxy-7-phosphoheptulonate synthase activity

Solanum tuberosum

60.0/8.9

27.9/7.3

62

31

11/71

87

Molybdenum cofactor sulfurase

Q655R6

lyase activity

Oryza sativa

92.9/7.1

27.2/5.2

60

12

6/22

136

Probable beta-1,3-galactosyltransferase 18

Q8RX55

galactosyltransferase activity

Arabidopsis thaliana

77.9/8.7

35.1/9.1

66

17

7/27

 

Protein folding, degradation and assembly

        

148

Anaphase-promoting complex subunit 2

Q8H1U5

ubiquitin protein ligase binding

Arabidopsis thaliana

98.4/4.8

43.5/7.9

64

12

7/20

153

U-box domain-containing protein 34

Q8S8S7

ubiquitin-protein ligase activity

Arabidopsis thaliana

91.7/9.1

39.6/6.9

63

18

12/51

171

Ubiquitin carboxyl-terminal hydrolase 6

Q949Y0

ubiquitin-specific protease activity

Arabidopsis thaliana

54.0/5.8

41.6/5.8

58

19

8/35

175

U-box domain-containing protein 34

Q8S8S7

ubiquitin-protein ligase activity

Arabidopsis thaliana

91.7/9.1

44.7/6.2

58

19

12/67

 

Transport

        

24

Magnesium transporter MRS2-8 OS

Q8H1G7

metal ion transmembrane transporter activity

Arabidopsis thaliana

43.1/5.3

13.6/4.4

58

22

6/34

54

Putative copper-transporting ATPase 3

Q9SH30

ATP binding

Arabidopsis thaliana

109.0/6.0

29.0/8.0

65

18

14/48

 

Stress Related

        

4

Ninja-family protein AFP4 OS

Q9S7Z2

protein binding

Arabidopsis thaliana

35.6/8.5

7.1/5.0

57

40

9/70

9

Glutathione S-transferase 6

Q96266

glutathione binding

Arabidopsis thaliana

29.2/8.5

11.9/6.2

58

25

5/46

20

Annexin D6 OS

Q9LX08

calcium ion binding

Arabidopsis thaliana

36.6/7.7

14.0/5.1

62

20

7/39

41

Monodehydroascorbate reductase, chloroplastic

P92947

monodehydroascorbate reductase (NADH) activity

Arabidopsis thaliana

53.5/8.1

16.2/5.7

63

26

8/76

154

Monodehydroascorbate reductase

Q40977

monodehydroascorbate reductase (NADH) activity

Pisum sativum

47.3/5.8

43.5/6.8

58

22

7/31

 

Development

        

2

Pentatricopeptide repeat-containing protein At3g06430, chloroplastic OS

Q9SQU6

N/A

Arabidopsis thaliana

56.2/7.8

9.5/6.7

58

25

11/59

11

Protein BRUSHY 1

Q6Q4D0

protein binding

Arabidopsis thaliana

148.8/5.5

12.0/5.9

63

24

21/71

38

Protein PAIR1

Q75RY2

 

Oryza sativa

53.8/9.8

17.2/5.5

66

19

10/34

68

1-aminocyclopropane-1-carboxylate synthase 7

Q9STR4

1-aminocyclopropane-1-carboxylate synthase activity

Arabidopsis thaliana

51.0/5.9

26.2/6.3

57

19

10/72

 

Unknown

        

12

Probable protein ABIL5 OS

Q5JMF2

N/A

Oryza sativa

28.3/8.3

15.2/7.6

59

40

7/42

16

Pentatricopeptide repeat-containing protein At4g26800 OS

Q9SZ20

N/A

Arabidopsis thaliana

58.4/9.1

16.1/6.8

64

32

12/49

31

Putative F-box protein At1g20795 OS

Q9LM74

N/A

Arabidopsis thaliana

48.5/8.6

20.3/5.1

60

21

8/55

48

Probable protein ABIL5 OS

Q5JMF2

N/A

Oryza sativa

28.2/8.2

21.5/6.9

59

40

7/42

67

BRCT domain-containing protein At4g02110

O04251

N/A

Arabidopsis thaliana

142.5/8.4

28.4/7.5

59

19

18/80

108

Thylakoid lumenal 15.0 kDa protein 2, chloroplastic

Q9LVV5

N/A

Arabidopsis thaliana

24.7/5.7

37.8/5.8

68

26

7/37

113

Pentatricopeptide repeat-containing protein At2g01860

Q5XET4

N/A

Arabidopsis thaliana

56.0/9.2

36.1/6.8

59

39

15/95

  1. aSpot number as in Figure 2. bSwissprot database accession number. cMolecular functions were inferred from those reported in the UniProt database. dTheoretical molecular mass and isoelectric point. eExperimental molecular mass and isoelectric point. fMascot score. gSequence coverage. hNumber of matched and unmatched peptides.
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