Identification of maturation and protein synthesis related proteins from porcine oocytes during in vitro maturation

Table 1 Proteins down-regulated in the M II stage during IVM.

Spot No.	Protein name	NCBI Accession No.	Swiss-prot Accession No.	Type of analysis	PMF (MS)		Theoretical		P-value
					Score	% Coverage	MW [Da]	pI
1	similar to G patch domain and KOW motifs, partial	gi\|114688528		MALDI-TOF	74	45	23,984	6.54	0.031
2	Heat shock 70 kDa protein 5 glucose-regulated protein precursor (GRP 78) isoform 1	gi\|114626692		MALDI-TOF/TOF	123	32	68,354	5.15	0.031
3	TD and POZ domain-containing protein 1	gi\|57013061		MALDI-TOF	64	29	99,551	5.34	0.003
4	protein disulfide isomerase Protein disulfide-isomerase A3 precursor (ERP57)	gi\|860986		MALDI-TOF	84	24	57,043	6.1	0.008
5	M-phase phosphoprotein 1 (MPP1)	gi\|124015169	Q80WE4	MALDI-TOF	51	13	204,969	5.61	0.003
6	unnamed protein product	gi\|90076858		MALDI-TOF	98	32	34,313	6.04	0.017
7	Chain A, Steric And Conformational Features Of The Aconitase Mechanism	gi\|157829964		MALDI-TOF	87	26	86,449	8.24	0.003
8	Zona pellucida sperm-binding protein 3 precursor (Zona pellucida glycoprotein ZP3, Sperm receptor)	gi\|1353191	P42098	LC-MSMS	51	7	44,959	6.17	0.127
9	zona pellucida glycoprotein 4	gi\|47522906	Q07287	LC-MSMS	65	3	59296	8.47	0.011
10	Cerebellar degeneration-related protein 2	gi\|67460136		LC-MSMS	155	12	52068	4.94	0.027
11	peroxiredoxin 3	gi\|27806083	Q9BGI3	LC-MSMS	204	21	28,117	7.15	0.01
12	heat shock protein 27 kDa	gi\|50916342	Q5IZV0	LC-MSMS	75	20	14,211	5.94	0.004

ISSN: 1477-5956