Identification of maturation and protein synthesis related proteins from porcine oocytes during in vitro maturation

Table 2 Proteins up-regulated in the M II stage during IVM.

Spot No.	Protein name	NCBI Accession No.	Swiss-prot Accession No.	Type of analysis	PMF (MS)		Theoretical		P-value
					Score	% Coverage	MW [Da]	pI
13	protein kinase 5'-AMP-activated protein kinase subunit beta-1 [Fragment]	gi\|984249	P80387	MALDI-TOF/TOF	107	25	92,742	4.75	0.007
14	Myomegalin phosphodiesterase 4D interacting protein	gi\|114558492	Q5TB27	MALDI-TOF	72	14	165,585	5.36	0.011
15	major vault protein similar to lung resistance-related protein homologue	gi\|19913410	Q14764	MALDI-TOF/TOF	71	29	99,551	5.34	0.008
16	Heat shock protein HSP 90-alpha 2	gi\|61656603	Q14568	LC-MSMS	229	7	98,052	5.09	0.034
17	heat-shock protein hsp86	gi\|194033	P07901	LC-MSMS	151	19	35,652	4.56	0.034
18	heat shock protein 70.2	gi\|47523308	Q6S4N2	MALDI-TOF	73	26	70,340	5.6	0.013
19	phosphoglucomutase 5	gi\|156121315	A1L598	MALDI-TOF	72	26	62,708	6.77	0.007
20	Dystrobrevin alpha (DTNA)	gi\|13626507	Q9Y4J8	MALDI-TOF	61	23	84,679	6.37	0.02
21	cytoskeletal beta actin	gi\|45269029		LC-MSMS	251	19	44,763	5.55	0.79
22	spermine synthase (SPSY)	gi\|78369282	P52788	LC-MSMS	171	15	41,198	4.96	0.51
23	galactokinase I	gi\|3603423	P51570	LC-MSMS	54	3	42246	6.04	0.014
24	galactokinase 1	gi\|3603423	P51570	LC-MSMS	54	3	42,246	6.04	0.014
25	transferase, HG -phosphoribosyl	gi\|224052		LC-MSMS	107	11	24,362	6.24	0.015
26	glutathione S-transferase, mu 2	gi\|116047849	P08010	LC-MSMS	171	19	22,353	5.52	0.038
27	glutathione-S-transferase, mu 5	gi\|25282395		LC-MSMS	62	7	22353	5.52	0.019
28	Peroxiredoxin-2 (Thioredoxin peroxidase 1)	gi\|1717797	P52552	LC-MSMS	156	34	14,158	4.7	0.003

ISSN: 1477-5956