Table 1 Summary of immunoreactive proteins identified with convalescent sera from swine naturally infected APP serotype 1

APs1

gi|307245438

Polyribonucleotide nucleotidyltransferase

77.439/4.96

30.6/6.35

41 (48%)

Cytoplasmic

9.97

336

APs2

gi|190150916

ATP-binding protein

24.383/8.52

40.1/6.50

12 (59%)

Unknown

122

APs3

gi|3913235

Chaperonin GroEL (HSP60 family)

57.722/4.90

63.9/4.67

21 (35%)

Cytoplasmic

9.97

197

APs4

gi|126207624

CTP synthetase

60.032/5.70

60.1/4.70

31 (71%)

Cytoplasmic

9.97

424

APs5

gi|190151264

Chaperone protein dnaK

67.899/4.76

43.5/4.74

41 (64%)

Cytoplasmic

9.97

389

APs7

gi|126209221

fumarate hydratase

50.756/6.14

76.2/5.04

23 (43%)

Cytoplasmic

9.97

157

APs8

gi|303252313

bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

61.029/6.31

61.2/5.08

35 (60%)

Periplasmic

9.76

259

APs10

gi|307245892

Transketolase 2

73.746/5.47

64.2/5.56

36 (42%)

Cytoplasmic

9.97

297

APs11

gi|53728830

Translation elongation factors (GTPases)

77.515/5.10

94.6/5.11

41 (67%)

Cytoplasmic

9.97

356

APs12

gi|46143894

TPR repeat

55.365/9.45

97.6/5.54

28 (62%)

Unknown

373

APs13

gi|46143698

Sugar transferases involved in lipopolysaccharide synthesis

44.335/9.35

97.8/5.52

18 (59%)

CytoplasmicMembrane

10

268

APs14

gi|307245832

hypothetical protein appser1_10340

45.695/6.34

71.7/5.20

18 (55%)

Unknown

245

APs15

gi|303253793

Type I restriction enzyme EcoEI R protein

90.370/6.55

94.1/5.14

40 (59%)

Unknown

393

APs16

gi|307244854

GTP-binding protein typA/bipA

68.343/5.24

69.5/5.26

34 (42%)

CytoplasmicMembrane

7.88

284

APs17

gi|307246005

hypothetical protein appser1_12060

95.894/5.38

94.8/5.36

36 (45%)

Cytoplasmic

9.97

322

APs18

43 (54%)

379

APs19

gi|46143487

Asparagine synthetase A

37.397/5.91

76.1/5.53

21 (65%)

Cytoplasmic

10

290

APs20

gi|53729159

BioD-like N-terminal domain of phosphotransacetylase

77.017/5.50

74.9/5.55

40 (52%)

Cytoplasmic

9.97

384

APs21

gi|126207895

protective surface antigen D15 precursor

89.088/6.30

89.1/6.31

36 (47%)

OuterMembrane

10

302

APs22

gi|307245243

Protective surface antigen D15

89.172/6.30

89.2/6.22

21 (28%)

OuterMembrane

10

152

APs23

gi|303252337

DNA topoisomerase III

72.857/9.07

96.9/5.18

40 (72%)

Cytoplasmic

9.97

385

APs25

APs26

gi|60476777

ApxIIA

102.466/5.50

102.4/5.51

102.4/5.24

32 (35%)

13 (14%)

Extracellular

10

314

117

APBs1

APBs2

APBs3

APBs8

gi|307246344

Outer membrane protein P5

39.638/9.24

39.5/9.31

39.5/9.25

39.7/9.18

34.9/9.31

26 (72%)

24 (61%)

22 (55%)

28 (72%)

OuterMembrane

10

243

220

229

278

APBs4

APBs5

APBs6

gi|307246787

Outer membrane protein P5

38.778/9.51

39.2/9.52

39.2/9.56

39.2/9.62

26 (63%)

25 (76%)

30 (68%)

OuterMembrane

10

260

262

289

APBs7

gi|165976709

ABC-type transport system involved in resistance to organic solvents, auxiliary component

23.399/9.65

23.4/9.60

14 (34%)

Unknown

168

APBs9

gi|46143830

Periplasmic component of the Tol biopolymer transport system

44.768/8.97

48.8/8.56

26 (53%)

Periplasmic

9.76

310