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Table 2 Summary of immunoreactive proteins identified with hyperimmune sera raised in an S259-immunized rabbit

From: Immunoproteomic analysis of bacterial proteins of Actinobacillus pleuropneumoniae serotype 1

Spot No.

Protein No.

Protein Identified

Theoretical MW/pI

Experimental MW/pI

Peptide match (sequence coverage, %)

PSORTb localization

PSORTb Probabilitya

Mowse scoreb

APr4

gi|32034816

Ribosomal protein L7/L12

12.322/4.72

10.8/4.75

10 (99%)

Unknown

 

109

APr8

APr9

gi|165977457

putative aldehyde dehydrogenase

54.094/5.48

54.4/5.46

54.5/5.44

13 (29%)

26 (48%)

Cytoplasmic

9.26

122

221

APr10

APr11

gi|165976190

pyruvate dehydrogenase subunit E1

98.894/5.46

98.1/5.50

19 (28%)

45 (53%)

Cytoplasmic

9.97

433

339

Apr12

gi|53728874

Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrA

48.573/5.91

50.9/5.97

26 (63%)

Cytoplasmic

9.97

305

Apr14

gi|32035558

Transaldolase

34.959/ 5.02

37.7/5.03

37 (62%)

Cytoplasmic

9.97

117

APBr1

APBr2

gi|190150781

outer membrane protein P5 precursor

39.588/9.17

39.6/9.28

35.9/9.28

18 (55%)

OuterMembrane

10

170

209

APBr3

gi|32034275

Outer membrane protein and related peptidoglycan-associated (lipo)proteins

38.708/9.51

38.7/9.53

7 (29%)

OuterMembrane

10

94

  1. (a) Localization predictions based on PSORT server http://www.psort.org/ evaluation. The values are localization probabilities (from 0 to 10).
  2. (b) Mowse score is the score based on the mowse algorithm in Mascot http://www.matrixscience.com. Protein scores > 83 are significant (p < 0.05).