Figure 7

N-terminal truncated variants of SAA-1α and SAA-2α in HDL. A. Four SAA-1α and SAA-2α isoforms were separated by 2-DE and analyzed by peptide mass fingerprinting after CNBr cleavage. (1) Full-length N-terminal peptide-mass detected in two of the isoforms identified as SAA-1α and SAA-2α; (2, 3) Truncated peptide masses (desArg (des-R), desArgSer (des-RS) and desArgSerPhePhe (des-RSFF)) detected in the two isoforms with an acidic charge shift in 2-DE compared to corresponding native variant (illustrated in figure 6). B. Three representative SELDI spectra demonstrating the presence of the SAA isoforms. Depicted are raw spectra of three different individuals before (green lines and 24 hours after (black lines) LPS infusion. The different SAA isoforms are indicated by the subsequent dashed vertical lines; a and b; des-RSFF of SAA-2α and -1α, c and d; des-RS of SAA-2α and -1α; e and f; des-R of SAA-2α and -1α and g and h; Native SAA-2α and -1α, respectively.