Analysis of EGFR signaling pathway in nasopharyngeal carcinoma cells by quantitative phosphoproteomics

Ruan, Lin; Li, Xin-Hui; Wan, Xun-Xun; Yi, Hong; Li, Cui; Li, Mao-Yu; Zhang, Peng-Fei; Zeng, Gu-Qing; Qu, Jia-Quan; He, Qiu-Yan; Li, Jian-Huang; Chen, Yu; Chen, Zhu-Chu; Xiao, Zhi-Qiang

doi:10.1186/1477-5956-9-35

Proteome Science

Table 1 Identification of differential phosphoproteins in EGF-stimulated and unstimulated NPC CNE2 cells by 2-D DIGE and MS

From: Analysis of EGFR signaling pathway in nasopharyngeal carcinoma cells by quantitative phosphoproteomics

No.	Protein name	Gene name	Uniprot accession	Mw (Da)	p I	Number of Matched	Sequence coverage	Score	Ratio (experiment/control)		Phosphorylation sites
						peptides	(%)		Ratio	t test
1	Translation endoplasmic reticulam ATPase (TERA)	VCP	P55072	89950	5.14	16	31%	148	1.75	0.0082	Yes
2	Heat shock cognate 71kDa protein	HSP7C	P11142	71082	5.37	13	38%	118	-1.51	0.018	Yes
3	Keratin,type ‖ cytoskeletal 8	KRT8	P05787	53671	5.52	18	43%	139	3.47	0.0035	Yes
4	BAG family molecular chaperone regulator 5	BAG5	Q9UL15	51738	5.76	4	15%	56	-2.51	0.0001	No
5	TUBB protein	TUBB	P07437	50095	4.78	25	62%	278	3.12	0.0009	Yes
6	Vimentin	VIM	P08670	53676	5.06	12	30%	102	1.99	0.0056	Yes
7	Heterogeneous nuclear ribonucleoprotein K	hnRNPK	P61978	51230	5.39	8	31%	86	2.89	0.0005	Yes
8	60KD Heat shock protein	HSP60	P10809	61187	5.70	12	29%	109	2.20	0.008	Yes
9	Keratin,type ‖ cytoskeletal 8	KRT8	P05787	53671	5.52	6	36%	85	1.63	0.032	Yes
10	Proliferating cell nuclear antigen	PCNA	P12004	29092	4.57	9	52%	93	2.88	0.0001	Yes
11	F-actin-capping protein subunit beta	CAPZB	P47756	31616	5.36	5	26%	56	2.23	0.009	Yes
12	laminin receptor 1	RSSA	P08865	32947	4.79	7	38%	76	1.76	0.0056	Yes
13	Creatine kinase B-type	CKB	P12277	42902	5.34	7	38%	100	1.99	0.0021	Yes
14	Emerin	EMD	P50402	28994	5.29	6	21%	77	1.71	0.0014	Yes
15	Heterogeneous nuclear ribonucleoproteins C1/C2	hnRNP C1/C2	P07910	33707	4.95	5	18%	68	2.98	0.0008	Yes
16	ATP synthase D chain mitochondrial	ATP5H	O75947	18537	5.21	5	37%	57	2.67	0.005	Yes
17	Prohibitin	PHB	P35232	29843	5.57	7	37%	120	-2.54	0.031	Yes
18	unidentified								1.79	0.002	N/A
19	Elongation factor 1- delta	EEF1D	P29692	32.217	4.9	4	25%	72	1.81	0.026	Yes
20	Anamorsin	CIAPIN1	Q6FI81	34141	5.44	9	39%	139	2.90	0.0001	Yes
21	Annexin A3	ANXA3	P12429	36524	5.63	7	27%	83	2.83	0.0054	Yes
22	Glutathione S-transferase P1	GSTP1	P09211	23569	5.43	6	34%	97	3.11	0.0013	Yes
23	unidentified								1.64	0.026	N/A
24	Keratin,type∣cytoskeletal 18	KRT18	P05783	48029	5.34	13	36%	141	-2.86	0.029	Yes
25	Ran-specific GTPase- activating protein	RANBP1	P43487	23310	5.71	6	32%	98	1.89	0.034	Yes
26	unidentified								1.68	0.007	N/A
27	Tropomodulin-3	TMOD3	Q9NYL9	39.595	5.08	8	34%	98	2.61	0.003	Yes
28	Myosin light polypeptide 6	MYL6	P60660	17090	4.56	6	50%	70	2.80	0.0019	Yes
29	Nucleoside diphosphate kinase A (NDKA)	NME1	P15531	17309	5.83	6	48%	105	-1.56	0.0023	Yes
30	Heat shock protein beta-1	HSP27	P04792	22826	5.98	7	47%	84	2.65	0.0001	Yes
31	unidentified								-2.72	0.038	N/A
32	Growth factor receptor-bound protein 2	GRB2	P62993	25,206	5.89	7	39%	75	2.60	0.00028	Yes
33	Glutathione S-transferase P1	GSTP1	P09211	23569	5.43	6	43%	91	3.99	3.2E-06	Yes
34	Peroxiredoxin-2	PRDX2	P32119	22049	5.66	6	54%	98	-1.69	0.042	Yes
35	c-Myc-responsive protein	RCL	O43598	19211	4.97	4	25%	67	2.25	0.009	Yes
36	Protein DJ-1	PARK7	Q99497	19.891	6.33	5	30%	65	2.99	0.0036	Yes
37	3-Hydroxyisobutyrate dehydrogenase mitochondrial precursor (3HIDH)	HIBADH	P31937	35705	8.38	4	22%	95	-2.55	0.0051	Yes
38	Stathmin	STMN1	P16949	17171	5.77	6	19%	95	2.57	0.0023	Yes

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ISSN: 1477-5956

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