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Table 1 Proteins identified in the V0 fraction of hAECS following SDS-PAGE.

From: Analysis of the proteome of human airway epithelial secretions

Gene Name

Protein Name

Gel Slice a

Ion Score b

Mol Wt (Da) c

Matches d

A2AP_HUMAN

Alpha-2-antiplasmin

3-4

57

54,531

1

A2MG_HUMAN

Alpha-2-macroglobulin

2

134

163,175

3

AACT_HUMAN

Alpha-1-antichymotrypsin

4

240

47,621

6

ANXA1_HUMAN

Annexin A1

5

245

38,690

7

ANXA2_HUMAN

Annexin A2

5

539

38,580

14

CEAM5_HUMAN

Carcinoembryonic antigen-related cell adhesion molecule 5

2-3

75

76,748

1

CATD_HUMAN

Cathepsin D

4

102

44,524

6

CD59_HUMAN

CD59 glycoprotein

9-10

121

14,168

5

CERU_HUMAN

Ceruloplasmin

2

85

122,128

3

CLUS_HUMAN

Clusterin

3-4

167

52,461

3

CFAH_HUMAN

Complement factor H

2

58

139,005

1

CFAI_HUMAN

Complement factor I

3

64

65,677

1

CO3_HUMAN

Complement C3

1

1,453

187,030

38

CYTB_HUMAN

Cystatin-B

10

91

11,133

2

DDEF2_HUMAN

Development and differentiation-enhancing factor 2

2

45

111,581

2

ENOA_HUMAN

Alpha-enolase (Enolase 1)

4

110

47,139

2

EZRI_HUMAN

Ezrin

3

105

69,370

2

FBLN3_HUMAN

EGF-containing fibulin-like extracellular matrix protein 1

3-4

39

54,604

1

FETUA_HUMAN

Alpha-2-HS-glycoprotein (Fetuin A)

5

142

39,300

3

ALDOA_HUMAN

Fructose-bisphosphate aldolase A

5

76

39,395

1

LG3BP_HUMAN

Galectin-3-binding protein

3

120

65,289

5

GELS_HUMAN

Gelsolin

2

293

85,644

10

G3P_HUMAN

Glyceraldehyde-3-phosphate dehydrogenase

5

187

36,030

4

GSTM1_HUMAN

Glutathione S-transferase Mu 1

6

128

25,695

6

GSTM2_HUMAN

Glutathione S-transferase Mu 2

6

168

25,728

6

GSTM3_HUMAN

Glutathione S-transferase Mu 3

6

180

26,542

9

GSTM5_HUMAN

Glutathione S-transferase Mu 5

6

172

25,658

7

GSTMP1_HUMAN

Glutathione S-transferase P

6-7

53

23,341

1

HBA_HUMAN

Hemoglobin subunit alpha

9

56

15,248

1

INADL_HUMAN

InaD-like protein

1

53

196,247

1

ITIH2_HUMAN

Inter-alpha-trypsin inhibitor heavy chain H2

2

112

106,370

2

LDHA_HUMAN

L-lactate dehydrogenase A chain

5

131

36,665

3

LDHB_HUMAN

L-lactate dehydrogenase B chain

5

114

36,615

2

TRFL_HUMAN

Lactotransferrin (Lactoferrin)

2

100

78,132

7

LPLC1_HUMAN

Long palate, lung and nasal epithelium clone protein 1

3-4

1,188

52,408

36

MUC1_HUMAN

Mucin-1

1

131

121,999

3

MUC4_HUMAN

Mucin-4

1

131

231,440

3

MUC5B_HUMAN

Mucin-5B

1

1,207

590,122

27

MUC16_HUMAN

Mucin-16

1

56

2,351,813

2

NGAL_HUMAN

Neutrophil gelatinase-associated lipocalin

7

344

22,574

12

PRDX1_HUMAN

Peroxiredoxin-1

7

125

22,096

4

PEDF_HUMAN

Pigment epithelium-derived factor

4

187

46,313

5

PIGR_HUMAN

Polymeric immunoglobulin receptor

2

999

83,232

24

PLUNC_HUMAN

Protein PLUNC

6

438

26,696

13

S100P_HUMAN

Protein S100-P (S100 calcium binding protein P)

10

118

10,393

3

S10A6_HUMAN

Protein S100-A6 (S100 calcium binding protein A6)

10

48

10,173

2

S10A8_HUMAN

Protein S100-A8 (S100 calcium binding protein A8)

10

61

10,828

2

S10AB_HUMAN

Protein S100-A11 (S100 calcium binding protein A11)

10

41

11,733

1

S10AG_HUMAN

Protein S100-A16 (S100 calcium binding protein A16)

10

42

11,794

1

RAB1A_HUMAN

Ras-related protein Rab-1A

7-8

100

22,663

2

RAB1B_HUMAN

Ras-related protein Rap-1B

7-8

118

20,812

3

TIMP1_HUMAN

Tissue inhibitor of metalloproteinase 1

7

74

23,156

1

UB2D2_HUMAN

Ubiquitin-conjugating enzyme E2 D2

9

40

16,724

2

UB1Q_HUMAN

Ubiquitin (ribosomal protein S27a)

10

154

8,560

3

VTDB_HUMAN

Vitamin D-binding protein

3-4

142

52,929

2

  1. aSDS-PAGE gel slice number (1 [top] - 10 [bottom]) from which the protein was isolated.
  2. bIon Score, probability value that an observed peptide match is a true match; cuttoff value = 10.
  3. cTheoretical molecular weight from Swiss-Prot database.
  4. dNumber of tryptic peptides generated that match the mass of theoretical trypsin peptides.
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Proteome Science

ISSN: 1477-5956