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Figure 1 | Proteome Science

Figure 1

From: Proteomic analysis of pregnancy-related proteins from pig uterus endometrium during pregnancy

Figure 1

Representative silver-stained 2D gel images of the different developmental stages of porcine endometrium. Proteins isolated from e NP, e 40, e 70 and e 93 with 200 μg of total protein loaded into the 2-DE gel. First dimension: 18 cm, with a pH of 3 to 11 linear IPG; second dimension: 12% (A, e NP; B, e 40; C, e 70; D, e 93). Among 3,000 spots visualized using silver staining, 53 proteins were identified from 12% and 8% gels (Figure 1 and 2). Protein spots, which changed by more than twofold compared to the e NP control, were marked and identified using MALDI-TOF and MALDI-TOF/TOF MS.

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