Figure 5

Enlarged images of differentially expressed protein spots related to chaperoning and anti-apoptotic function and confirmation of proteins by western blotting among e NP, e 40, e 70 and e 93. (A) Different expression of Hsp 90β and Hsp 27 during different developmental stages of porcine endometrium. (B) The amount of protein expression was measured by scanning and the TINA analysis program. (C) Confirmation of differentially expressed proteins in different developmental stages of porcine endometrium by western blot analysis (Hsp 90α, Hsp 90β, Hsp70, Hsp60 and Hsp27 proteins). β-tubulin was used as an internal control. (D) Quantification of Hsp 90α, Hsp 90β, Hsp70, Hsp60 and Hsp27 protein expression during porcine pregnancy.