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Figure 3 | Proteome Science

Figure 3

From: Antibody cross-linking and target elution protocols used for immunoprecipitation significantly modulate signal-to noise ratio in downstream 2D-PAGE analysis

Figure 3

SDS elution from Dynabeads® protein A with 2% SDS is fully compatible with downstream 2DE. Eluted HeLa cell proteins from beads cross-linked to non-immune mouse IgG (A), anti-PCNA using BS3 as cross-linker (B), or anti-PCNA using DMP as cross-linker (C). Samples were used to rehydrate 7 cm IPG strips, pH 4-7 overnight. Proteins were stained using Sypro Ruby. PCNA spots are indicated by arrows.

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Proteome Science

ISSN: 1477-5956