Skip to main content
Figure 4 | Proteome Science

Figure 4

From: Antibody cross-linking and target elution protocols used for immunoprecipitation significantly modulate signal-to noise ratio in downstream 2D-PAGE analysis

Figure 4

Choice of elution buffer is crucial for identification of non-abundant proteins. 2DE protein patterns after IP performed with beads cross-linked to anti-normal rabbit IgG (A) or anti-XRCC1 antibody (B-F) using BS3. Bound proteins were eluted using SDS (A,B), glycine (C), KCl-HCl (D), Destreak (E) and urea-CHAPS (F) buffers. Proteins were stained using Sypro Ruby.

Back to article page

Proteome Science

ISSN: 1477-5956