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Table 1 XRCC1 was identified by MALDI peptide mass fingerprinting subsequent to elution from Protein A beads with SDS, Urea-CHAPS or Destreak solution.

From: Antibody cross-linking and target elution protocols used for immunoprecipitation significantly modulate signal-to noise ratio in downstream 2D-PAGE analysis

Elution buffer

Mascot score

Number of peptides

SDS

205

15

Urea-CHAPS

118

10

Destreak

67

7

  1. SDS elution yielded significantly better score value and higher number of identified peptides than the other two elution buffers.
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