Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE

Table 2 2-DE spots that increased significantly with PAF

Spot Number	Protein Sequence ¹
60	Omega-gliadin Gli-B3 TC11_288652²
63	Omega-gliadin Gli-B3 TC11_288652²
71	Omega-gliadin Gli-B3 TC11_288652²
74	Omega-gliadin Gl-iB3 TC11_288652²
69	Omega-gliadin Gli-B3³
73	Omega-gliadin Gli-B3³
107	Omega-gliadin Cys type TC_262770²
113	Omega-gliadin Cys type TC_262770⁴
115	Omega-gliadin Cys type TC_262770⁴
135	Omega-gliadin Gli-A3 Bu-D5²
391	Omega-gliadin Gli-A3 Bu-D5⁵
476	Omega-gliadin Gli-D3³
477	Omega-gliadin Gli-D3 [GenBank:AAT74547]⁵
12	HMW-GS Ax2* [GenBank:AAB02788]²
17	HMW-GS Ax2* [GenBank:AAB02788]²
19	HMW-GS Ax2* [GenBank:AAB02788]²
20	HMW-GS Bx7 [GenBank:CAA32115]²
21	HMW-GS Bx7 [GenBank:CAA32115]²
302	HMW-GS Bx7 [GenBank:CAA32115]²
350	HMW-GS 1By9 [GenBank:CAA43361]²
3	HMW-GS Dx5 [GenBank:ABG68042]²
7	HMW-GS Dx5 [GenBank:ABG68042]²
10	HMW-GS Dx5 [GenBank:ABG68042]²
42	HMWGS Dy10 [GenBank:P10387]⁴
342	Alpha-gliadin Bu-1^5,6
468	Alpha-gliadin Bu-3^5,6
467	Alpha-gliadin Bu-4^5,6
327	Alpha-gliadin Bu-11⁵
387	Alpha-gliadin Bu-12⁵
524	Alpha-gliadin Bu-12⁵
190	Alpha-gliadin Bu-14⁴
124	Alpha-gliadin Bu-BQ807130^2,6
148	Serpin Bu-1²
149	Serpin Bu-2⁵
397	Serpin Bu-2²
398	Serpin Bu-3²
399	Serpin Bu-3²
159	Serpin Bu-4^4,6
151	Serpin Bu-5²
167	LMW-GS Bu-1⁵
131	LMW-GS Bu-3²
317	LMW-GS Bu-2/13⁴
318	LMW-GS Bu-2/13⁵
155	LMW-GS Bu-18⁵
325	Gamma-gliadin Bu-1or Bu-8^5,7
334	Gliadin, mixed spot⁸
348	Triticin TC11_285558⁵
176	Malate dehydrogenase [GenBank:AAT64932]²

¹ Identifications are from Dupont et al. [28].
² MS/MS analysis in [28] indicated that spot contained only one protein.
³ No peptides identified by MS/MS in [28]. Spot identified in [43].
⁴ Predominant protein identified by MS/MS in [28]. Analysis indicated that spot also contained other proteins from the same class.
⁵ Predominant protein identified by MS/MS in [28]. Analysis indicated that spot also contained proteins from a different class.
⁶ Predominant protein sequence identified in only one spot.
⁷ MS/MS data in [28] was not sufficient to discriminate among proteins.
⁸ More than one protein was identified in spot in [28], but no one protein was predominant.

ISSN: 1477-5956