Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE

Altenbach, Susan B; Tanaka, Charlene K; Hurkman, William J; Whitehand, Linda C; Vensel, William H; Dupont, Frances M

doi:10.1186/1477-5956-9-46

Proteome Science

Table 3 Individual 2-DE spots that decreased significantly with PAF

From: Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE

Spot Number	Protein Sequence ¹
289	Alpha-amylase inhibitor WMAI [PRF:223520]²
528	Alpha-amylase inhibitor WMAI [PRF:223520]²
283	Alpha-amylase inhibitor WDAI TC11_338524^3,4
286	Alpha-amylase inhibitor WDAI [GenBank:AAV91972]^3,4
312	Alpha-amylase inhibitor WDAI [SwissProt:P01085]^4,5
313	Alpha-amylase inhibitor WTAI-CM1 TC11_340510^4,5
280	Alpha-amylase inhibitor WTAI-CM2 [SwissProt:P16851]³
285	Alpha-amylase inhibitor WTAI-CM2 [SwissProt:P16851]³
264	Alpha-amylase inhibitor WTAI-CM3 [SwissProt:P17314]³
265	Alpha-amylase inhibitor WTAI-CM3 [SwissProt:P17314]³
266	Alpha-amylase inhibitor WTAI-CM16 [SwissProt:P16159]²
284	Alpha-amylase inhibitor WTAI-CM16 [SwissProt:P16159]²
274	Alpha-amylase inhibitor WTAI-CM17 [GenBank:CAA42453]²
282	Alpha-amylase inhibitor WTAI-CM17 [GenBank:CAA42453]³
244	Alpha-amylase/subtilisin inhibitor WASI [SwissProt:P16347]^2,4
281	Protease inhibitor CMx1/CMx3 TC11_309398^3,4
290	Protease inhibitor CMx1/CMx3 TC11_308146^3,4
277	Chymotrypsin inhibitor WCI [GenBank:CAD19440]^2,4
278	Chymotrypsin inhibitor WCI, mixed spot⁶
170	LMW-GS Bu-1⁵
119	LMW-GS Bu-3²
161	LMW-GS Bu-3⁵
237	LMW-GS Bu-3²
316	LMW-GS Bu-3⁵
140	LMW-GS Bu-4²
173	LMW-GS Bu-6^4,5
145	LMW-GS Bu-7³
203	LMW-GS Bu-11⁵
315	LMW-GS Bu-11³
153	LMW-GS Bu-18²
326	Gamma-gliadin Bu-1^4,5
166	Gamma-gliadin Bu4⁵
134	Gamma-gliadin Bu-5⁵
323	Gamma-gliadin Bu-5⁵
335	Gamma-gliadin Bu-6⁵
527	Gamma-gliadin Bu-11⁵
385	Farinin Bu-1²
193	Farinin Bu-2⁵
207	Farinin Bu-2²
549	Farinin Bu-2²
336	Farinin Bu-3⁵
345	Farinin Bu-3⁵
542	Purinin Bu-1⁵
543	Purinin Bu-1⁵
219	Purinin Bu-2²
223	Purinin Bu-2²
220	Purinin Bu-3²
227	Purinin Bu-3²
99	Globulin-2 Bu-17366^2,4
104	Globulin-2 Bu-17295²
106	Globulin-2 Bu-18428²
184	Globulin Glo-3 TC11_305389³
272	Globulin Glo-3 TC_234094⁵
14	HMW-GS Dx5 [GenBank:ABG68042]²
538	HMW-GS Dx5 [GenBank:ABG68042]²
25	HMW-GS Bx7 [GenBank:CAA32115]²
33	HMW-GS Bx7 [GenBank:CAA32115]²
514	HMWGS Dy10 [GenBank:P10387]²
94	Beta-amylase Bu-2³
93	Beta-amylase Bu-3³
232	Chitinase [GenBank:BAB18520]^2,4
455	Chitinase [GenBank:AAX83262.1]^2,4
248	Puroindoline-b [GenBank:AAT40244]³
271	Puroindoline-b [GenBank:AAT40244]²
89	ADP glucose pyrophosphorylase, large subunit [GenBank:CAD98749]²
295	Lipid transfer protein (LTP) Bu-2^2,4
456	Thaumatin-like protein TC11_283136^2,4
225	Triose phosphate isomerase [GenBank:CAC14917]^2,4
311	Elongation factor EF1A [SwissProt:Q03033]^2,4
202	Glucose and ribitol dehydrogenase RS_UWI_14903^2,4
195	Gliadin, mixed spot⁶

¹ Identifications are from Dupont et al. [28].
² MS/MS analysis in Dupont et al. [28] indicated that spot contained only one protein.
³ Predominant protein identified by MS/MS in Dupont et al. [28]. Analysis indicated that spot also contained other proteins from the same class.
⁴ Predominant protein sequence identified in only one spot.
⁵ Predominant protein identified by MS/MS in [28]. Analysis indicated that spot also contained proteins from a different class.
⁶ More than one protein was identified in spot, but no one protein was predominant.

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ISSN: 1477-5956

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