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Table 4 Unique proteins that changed significantly with PAF

From: Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE

   

Spot Volume

 

Protein Sequence 1

# Spots

Spot Numbers 2

-PAF

+PAF

% Change

Omega-gliadin Gli-B3 type3

6

60+, 63+, 69+,71+, 73+, 74+

1.940

5.070

161.4

Omega-gliadin Gli-D3 type4

2

476+, 477+

1.413

3.059

116.5

Omega-gliadin Bu-D5

2

135+, 391+

0.392

0.985

151.4

Omega-gliadin Cys type TC_262770

4

107+, 113+, 115+, 116

0.502

1.248

148.4

HMW-GS Ax2* [GenBank:AAB02788]

8

12+, 16, 17+, 18, 19+, 23, 24, 28

1.719

2.411

40.2

HMW-GS Bx7 [GenBank:CAA32115]

8

20+, 21+, 25-, 26, 27, 29, 33-, 302+

3.885

5.019

29.2

HMW-GS Dx5 [GenBank:ABG68042]

9

3+, 5, 7+, 10+, 14-, 36, 138, 307, 538-

2.691

3.732

38.7

HMW-GS 1By9 [GenBank:CAA43361]

8

40, 47, 52, 358, 349, 350+, 351, 360

1.478

2.277

54.0

HMWGS Dy10 [GenBank:P10387]

7

42+, 48, 51, 333, 407, 424, 514-

3.100

3.675

18.6

Alpha-gliadin Bu-1

1

342+

0.516

0.845

63.8

Alpha-gliadin Bu-3

1

468+

1.219

1.658

36.0

Alpha-gliadin Bu-4

1

467+

1.296

2.006

54.7

Alpha-gliadin Bu-12

5

328, 329, 387+, 524+, 525

1.903

3.063

60.9

Alpha-gliadin Bu-14

4

331, 190+, 206, 546

0.821

1.094

33.2

Alpha-gliadin Bu-BQ807130

1

124+

0.188

0.287

52.6

Serpin Bu-1

3

146, 147, 148+

0.461

0.599

30.1

Serpin Bu-1 or Bu-4

2

158, 162

0.069

0.040

-41.3

Serpin Bu-2

2

149+, 397+

0.067

0.193

188.6

Serpin Bu-3

2

398+, 399+

0.038

0.087

126.1

Serpin Bu-4

1

159+

0.146

0.236

62.2

Serpin Bu-5

2

151+, 154

0.181

0.268

48.5

Alpha-amylase inhibitor WMAI [PRF:223520]

2

289-, 528-

1.043

0.492

-52.9

Alpha-amylase inhibitor WDAI TC11_338524

1

283-

0.357

0.216

-39.6

Alpha-amylase inhibitor WDAI [GenBank:AAV91972]

1

286-

0.274

0.115

-57.9

Alpha-amylase inhibitor WDAI [SwissProt:P01085]

1

312-

1.305

0.671

-48.6

Alpha-amylase inhibitor WTAI-CM1 TC11_340510

1

313-

0.557

0.210

-62.3

Alpha-amylase inhibitor WTAI-CM2 [Swiss-Prot:P16851]

2

285-, 280-

1.377

0.479

-65.2

Alpha-amylase inhibitor WTAI-CM3 [Swiss-Prot:P17314]

2

264-, 265-

1.409

0.495

-64.9

Alpha-amylase inhibitor WTAI-CM16 [Swiss-Prot:P16159]

2

266-, 284-

1.235

0.512

-58.6

Alpha-amylase inhibitor WTAI-CM17 [Genbank:CAA42453]

2

274-, 282-

0.634

0.217

-65.8

Alpha-amylase/subtilisin inhibitor WASI [SwissProt:P16347]

1

244-

0.264

0.171

-35.0

Protease inhibitor CMx1/CMx3 TC11_309398

1

281-

0.361

0.229

-36.5

Protease inhibitor CMx1/CMx3 TC11_308146

1

290-

0.074

0.028

-62.8

Chymotrypsin inhibitor WCI [GenBank:CAD19440]

1

277-

0.327

0.116

-64.7

Farinin Bu-1

3

196, 385-, 386

0.358

0.246

-31.2

Farinin Bu-2

3

193-, 207-, 549-

1.271

0.325

-74.4

Farinin Bu-3

2

336-, 345-

0.815

0.336

-58.7

Purinin Bu-1

2

542-, 543-

0.487

0.294

-39.7

Purinin Bu-2

2

219-, 223-

0.524

0.261

-50.2

Purinin Bu-3

2

220-, 227-

0.491

0.270

-45.0

LMW-GS Bu-3

9

119-, 119a, 120, 131+, 132, 161-, 237-, 310, 316-

8.015

6.062

-24.4

LMW-GS Bu-6

1

173-

0.634

0.471

-25.7

LMW-GS Bu-7

3

144, 145-, 472

3.320

2.473

-25.5

Gamma-gliadin Bu-1

1

326-

0.191

0.132

-30.9

Globulin-2 Bu-18428

2

103, 106-

0.129

0.088

-31.6

Globulin-2 Bu-17366

1

99

0.043

0.028

-34.1

Chitinase [GenBank:BAB18520]

1

232-

0.095

0.041

-56.8

Chitinase [GenBank:AAX83262.1]

1

455-

0.125

0.047

-62.3

Puroindoline b [Genbank:AAT40244]

2

248-, 271-

0.512

0.251

-51.0

Beta-amylase Bu-2

2

94-, 462

0.231

0.145

-37.4

Lipid transfer protein (LTP) Bu-2

1

295-

0.117

0.035

-69.8

Thaumatin-like protein TC11_283136

1

456-

0.095

0.049

-48.4

Triose phosphate isomerase [GenBank:CAC14917]

1

225-

0.081

0.046

-42.6

Elongation factor EF1A [SwissProt:Q03033]

1

311-

0.168

0.113

-32.7

Glucose and ribitol dehydrogenase RS_UWI_14903

1

202-

0.117

0.004

-96.6

  1. 1 Identifications are from Dupont et al. [28].
  2. 2 Spots that increased or decreased significantly (p < 0.02) are indicated with + and -, respectively.
  3. 3 Includes proteins identified as TC11_288652.
  4. 4 Includes protein identified as [GenBank:AAT74547].
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