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Figure 1 | Proteome Science

Figure 1

From: Comparison of human glomerulus proteomic profiles obtained from low quantities of samples by different mass spectrometry with the comprehensive database

Figure 1

Peptide and protein identification from low quantities of normal human glomeruli using five mass spectrometers. A. Protein digests of human glomeruli were analyzed on five mass spectrometers including LIT-TOF, LTQ-Orbitrap, Q-TOF, LIT and MALDI-TOF/TOF, yielding 139, 185, 94, 255, 108 proteins respectively (significance p < 0.01, FDR < 1%), and 521, 792, 320, 995 and 445 peptides respectively (ions score > identity threshold). An integrated profile of 332 proteins was subsequently generated. Blue column represents the number of proteins identified by all the MS instruments, while light blue by at least two (2~4) and white uniquely by only one. B. Protein distribution patterns for the integrated profile and the comprehensive one were plotted due to their physicochemical properties (pI and MW) and no discernible bias was observed. Gray circles, proteins in the comprehensive database; black spots, proteins in the integrated dataset. C. The total glomerular proteins identified in this study were categorized by protein class, ranking cytoskeletal proteins to the top with the largest proportion.

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Proteome Science

ISSN: 1477-5956