Figure 5

Western blot analyses of regulated proteins. Caco-2 cells were treated with rTcdA wt or mutant rTcdA (500 ng/mL) for 5 h or 24 h. After fractionation the cytosolic proteins were separated on 12% SDS-PAGE and probed with antibodies against regulated ptoteins. The western blot quantitation was performed on a Kodak Image Station 440 CF by analysis with the Kodak 1D Image software, for normalization of samples α-tubulin was used. The bars represent fold regulation factors referred to values of control samples (mean values + SD, n ≥ 3). The mass spectrometry data of corresponding proteins were shown to compare the results of this study. A) Endoplasmin (HSP90B1); B) Glucosidase 2 subunit beta (PRKCSH); C) Creatin kinase b-type (CKB); D) Apolipoprotein E (ApoE); E) Rac1; F) Clathrin, heavy chain 1 (CLH-17).