Impact of clostridial glucosylating toxins on the proteome of colonic cells determined by isotope-coded protein labeling and LC-MALDI

Jochim, Nelli; Gerhard, Ralf; Just, Ingo; Pich, Andreas

doi:10.1186/1477-5956-9-48

Proteome Science

Table 1 Regulated proteins in Caco-2 cells after treatment with rTcdA wt or mutant rTcdA using ICPL-LC-MS-technique

From: Impact of clostridial glucosylating toxins on the proteome of colonic cells determined by isotope-coded protein labeling and LC-MALDI

	after treatment with mutant rTcdA, 5 h
Nr.	Protein name	Accession number	Gene name	Number of peptides for quantitation	regulation factor ¹	SD ²	T-test ³
1	Acetyl-CoA acetyltransferase, cytosolic variant	Q9BWD1	ACAT 2	8	2.39	0.53	0.047
2	Alpha-enolase	P06733	ENO1	3	1.96	0.31	<0.0001
3	Annexin A3	P12429	ANXA3	5	1.59	0.22	0.015
4	Glucosamine--fructose-6-phosphate aminotransferase	Q06210	GFPT1	4	1.79	0.51	0.005
5	Macrophage migration inhibitory factor	P14174	MIF	6	1.59	0.35	0.042
6	Peroxiredoxin-1	Q06830	PRDX2	10	1.75	0.36	0.001
7	Peroxiredoxin-2	P32119	PRDX1	6	1.76	0.14	0.003
	after treatment with rTcdA wt, 5 h
Nr.	Protein name	Accession number	Gene name	Number of peptides for quantitation	regulation factor	SD	T-test
1	40S ribosomal protein S16	P62249	RPS16	3	0.38	0.07	0.01
2	40S ribosomal protein S17	P08708	RPS17	9	0.40	0.03	0.018
3	40S ribosomal protein S3	P23396	RPS3	5	0.35	0.02	0.004
4	40S ribosomal protein S9	P46781	RPS9	9	0.33	0.04	0.003
5	60S ribosomal protein L18	Q07020	RPL18	8	0.36	0.07	0.007
6	60S ribosomal protein L18a	Q02543	RPL18A	4	0.33	0.05	0.004
7	60S ribosomal protein L8	P62917	RPL8	5	0.22	0.02	0.024
8	60S ribosomal protein L9	P32969	RPL9	6	0.36	0.01	0.04
9	Glutamate dehydrogenase 1, mitochondrial	P00367	GLUD1	7	0.54	0.02	0.042
10	Heterogeneous nuclear ribonucleoprotein A1	P09651	HNRNPA1	8	0.50	0.11	0.024
11	Polypyrimidine tract-binding protein 1	P26599	PTBP1	5	0.57	0.2	0.03
12	Glucosidase 2 subunit alpha	Q14697	GANAB	19	1.76	0.13	<0.0001
13	Glucosidase 2 subunit beta	P14314	PRKCSH	6	1.95	0.04	0.038
14	Endoplasmin	P14625	HSP90B1	32	1.72	0.04	<0.0001
15	Protein disulfide-isomerase	P07237	P4HB	21	1.80	0.28	0.008
16	Protein disulfide-isomerase A4	P13667	PDIA4	10	1.65	0.3	0.0003
	after treatment with mutant rTcdA, 24 h
Nr.	Protein name	Accession number	Gene name	Number of peptides for quantification	regulation factor	SD	T-test
1	Filamin A	P21333	FLNA	4	0.7	0.06	0.015
	after treatment with rTcdA wt, 24 h
Nr.	Protein name	Accession number	Gene name	Number of peptides for quantification	regulation factor (average)	SD	T-test ³
1	Actin, cytoplasmic 1	P60709	ACTB	46	0.57	0.25	<0.0001
2	Filamin A	P21333	FLNA	4	0.52	0.04	0.01
3	Filamin B	O75369	FLNB	65	0.6	0.14	<0.0001
4	Villin-1	P09327	VIL1	11	0.58	0.19	0.008
5	Apolipoprotein E	P02649	APOE	4	0.29	0.00	0.041
6	Creatine kinase B-type	P12277	CKB	23	0.57	0.04	<0.0001
7	Glucosidase 2 subunit beta	P14314	PRKCSH	11	0.46	0.09	0.007
8	Peroxiredoxin-1	Q06830	PRDX1	22	0.72	0.14	<0.0001
9	Peroxiredoxin-2	P32119	PRDX2	15	0.63	0.11	0.004
10	Peroxiredoxin-6	P30041	PRDX6	6	0.58	0.04	0.026
11	Annexin A3	P12429	ANXA3	11	0.68	0.13	0.006

¹ The change in abundance of the proteins is expressed by the calculated ratio between with rTcdA wt or mutant rTcdA treated cells and control cells of all labeled peptides and the average ratio was determined.
² SD: standard deviation
³ T-test: Student's t-test was used for calculation of p-values, p < 0.05

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ISSN: 1477-5956

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